GAPDH

GAPDH

价格: ¥200

品牌:FLIGHT

货号:FLD-M-107

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供应商 :FLIGHT

库存 :100μg

靶点 :GAPDH

级别 :/

目录编号 :FLD-M-107

克隆性 :单克隆

抗原来源 :/

保质期 :1year

抗体英文名 :GAPDH

抗体名 :GAPDH

标记物 :/

宿主 :

适应物种 :H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh, Insect, Ye

免疫原 :/

亚型 :/

形态 :液体

应用范围 :WB,IHC, IF

保存条件 :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

浓度 :100μg/瓶

规格 :100μg

GAPDH mouse monoclonal antibody
Cat.#             
Package           100 μl
Storage           PBS with 0.1% sodium azide and 50% glycerol, pH 7.2

Product Imformation

Description        Anti-GAPDH mouse monoclonal antibody  
Applications       WB/ IHC/ IF
Immunogen       Fusion protein of human GAPDH
Reactivity          Human, Mouse, Rat
Host species       Mouse
Ig class            IgG1
Purity             Protein A/G Affinity Purification

Target information

Symbol           GAPDH 
Full name         glyceraldehyde-3-phosphate dehydrogenase    
Synonyms        G3PD; GAPD; HEL-S-162eP   
  

Target Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Western blotting 

Predicted band size     36 kDa
Positive control :        HUVEC cell and Human left lung tissue lysates
Recommended dilution :  1000-5000

Gel: 8%SDS-PAGE Lysate: 40 μg
Lane 1-2: HUVEC cell and Human left lung tissue lysates
Primary antibody: GAPDH Antibody at dilution 1/1200
Secondary antibody: Goat anti mouse IgG at 1/5000
dilution Exposure time: 3 minutes

 

福来德生物科技(武汉)有限公司

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入驻年限:3年

联系人:吴博士

地址:武汉

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