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供应商 :FLIGHT
库存 :100μg
靶点 :GAPDH
级别 :/
目录编号 :FLD-M-107
克隆性 :单克隆
抗原来源 :/
保质期 :1year
抗体英文名 :GAPDH
抗体名 :GAPDH
标记物 :/
宿主 :鼠
适应物种 :H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh, Insect, Ye
免疫原 :/
亚型 :/
形态 :液体
应用范围 :WB,IHC, IF
保存条件 :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
浓度 :100μg/瓶
规格 :100μg
GAPDH mouse monoclonal antibodyCat.#
Package 100 μl
Storage PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description Anti-GAPDH mouse monoclonal antibody
Applications WB/ IHC/ IF
Immunogen Fusion protein of human GAPDH
Reactivity Human, Mouse, Rat
Host species Mouse
Ig class IgG1
Purity Protein A/G Affinity Purification
Target information
Symbol GAPDH
Full name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD; GAPD; HEL-S-162eP
Target Background
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Western blotting
Predicted band size 36 kDa
Positive control : HUVEC cell and Human left lung tissue lysates
Recommended dilution : 1000-5000
Gel: 8%SDS-PAGE Lysate: 40 μg
Lane 1-2: HUVEC cell and Human left lung tissue lysates
Primary antibody: GAPDH Antibody at dilution 1/1200
Secondary antibody: Goat anti mouse IgG at 1/5000
dilution Exposure time: 3 minutes
福来德生物科技(武汉)有限公司
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入驻年限:3年