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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma(heparin, EDTA, citrate).
样本 ：Rat
规格 ：96T
产品概况
| 货号 | FEK1219 |
|---|---|
| 产品名称 | Rat MIP-1α Quick ELISA Kit |
| 产品描述 | For quantitative detection of rat MIP-1 alpha in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| 免疫原 | E.coli, A24-A92 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 7.8pg/ml-500pg/ml |
| 基因名称 | CCL3 |
| 基因全名 | chemokine (C-C motif) ligand 3 |
| Uniprot ID | CCL3: P50229 |
| 指标别名 | C C motif chemokine 3|CCL3|CCL3_HUMAN|Chemokine (C C motif) ligand 3|Chemokine ligand 3|G0/G1 switch regulatory protein 19 1|G0/G1 switch regulatory protein 19-1|G0S19 1|G0S19 1 protein|Heparin binding chemotaxis protein|L2G25B|LD78-alpha(4-69)|LD78ALPHA|Macrophage inflammatory protein 1 alpha|Macrophage inflammatory protein 1-alpha|MIP 1 alpha|MIP-1-alpha|MIP-1-alpha(4-69)|MIP1A|PAT 464.1|SCYA 3|SCYA3|SIS alpha|SIS beta|SIS-beta|Small inducible cytokine A3|Small-inducible cytokine A3|Tonsillar lymphocyte LD78 alpha protein |
| 背景资料 | Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. |
| 显色时间 | 15分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| FEK1219-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
| EK1219-ST | rat MIP-1α standard | 10ng/tube×2 |
| FEK1219-C | rat MIP-1α Antibody Cocktail | 6ml |
| AR1106-1 | Sample diluent buffer | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | S top solution | 10ml |
| PLA-SEA | Adhesive cover | 2 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 18 | 54 | 267 | 18 | 56 | 248 |
| Standard deviation | 1.08 | 3.61 | 15.75 | 1.11 | 4.48 | 14.88 |
| CV(%) | 6% | 6.7% | 5.9% | 6.2% | 8% | 6% |
Rat MIP-1α Quick ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15分钟)| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.021 | 0.125 | 0.220 | 0.377 | 1.710 | 1.249 | 1.954 | 2.369 |
武汉博士德生物工程有限公司
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入驻年限：17年
