Human Cytokine Release Syndrome (16-Plex)

Product Info Summary

Species	Human
Assay Type	Sandwich
Sample Type	Human Serum, EDTA/Heparin Plasma
Assay Length	4.83 hours
Specificity	All analytes < 1% intra-panel cross-reactivity
Volume Required Per Well	Min 25 ?l
Detection Method	Chemiluminescent
Multiplex Format	96-well solid plate
Catalog No	MEK2007
Manufacturer Reference Number:	119733HU
Analytes	IL-1b, IL-1Ra, IL-2, IL-2Ra, IL-6, IL-6r, IL-8, IL-10, IL12p70, IL-13, IFNy, IFN&alpha;, GM-CSF, TNF&alpha;, MCP-1, MIP1a

Product Overview

The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours. The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Assay Ranges

	Units	Calibrator Range	LLD Limit
GM-CSF	pg/ml	6,000-10.70	5.35
IFNα	pg/ml	400-0.72	0.36
IFNγ	pg/ml	99-0.18	0.09
IL-1β	pg/ml	4,075-7.27	3.64
IL-1Ra	pg/ml	7,000-12.48	6.24
IL-2	pg/ml	64-0.11	0.06
IL-2Ra	pg/ml	16,000-28.5	14.25
IL-6	pg/ml	333-0.59	0.3
IL-6R	pg/ml	1,250-2.23	1.11
IL-8	pg/ml	2,000-3.6	1.8
IL-10	pg/ml	2,680-4.78	2.39
IL-12p70	pg/ml	3,000-5.35	2.68
IL-13	pg/ml	333-0.59	0.3
MCP-1	pg/ml	2,000-3.6	1.8
MIP-1α	pg/ml	2,000-3.57	1.78
TNFα	pg/ml	661-1.18	0.59

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing.

Reagents Include:

Calibrator
Detection Mix
Substrate
Sample Diluents
Streptavidin HRP
Wash Buffer