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克隆性 ：Monoclonal(Clone:9F13E4）
适应物种 ：human, mouse, rat
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：WB, IHC, ICC/IF, FCM
规格 ：50μl/100μl/150μl
产品概况
| 货号 | M11793 |
|---|---|
| 产品名称 | Anti-MPPB/PMPCB Antibody (monoclonal, 9F13E4) |
| 基因名 | PMPCB |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:9F13E4） |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 43KD |
| 免疫原 | E.coli-derived human MPPB/PMPCB recombinant protein (Position: E23-Q479). |
| 内容 | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| 纯化方式 | protein G/A purified |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Mitochondrial-processing peptidase subunit beta is an enzyme that in humans is encoded by the PMPCB gene. This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex. |
| 研究类别 | 1. Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. (Letter) Nature 340: 245-246, 1989. 2. Gordon, D. M., Shi, Q., Dancis, A., Pain, D. Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase. Hum. Molec. Genet. 8: 2255-2262, 1999. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/3/2018. |
| Uniprot ID | PMPCB: O75439 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow Cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PMPCB antibody (M11793). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: HCCT whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates.
Use mouse anti- MPPB 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MPPB at approximately 43KD. The expected band size for MPPB is at 54KD.|Figure 2. IHC analysis using anti- PMPCB antibody (M11793). detected in paraffin-embedded section of human Ovarian Serous Carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- PMPCB antibody (M11793). detected in paraffin-embedded section of human adenocarcinoma of lung tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. ICC analysis using anti- PMPCB antibody (M11793). was detected in immersion fixed MCF7 cell. Cells were stained using the Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126) .and counterstained with DAPI (blue).|Figure 5. Flow cytometry analysis of JK cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: MCF-7 whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: HCCT whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates.
Use mouse anti- MPPB 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MPPB at approximately 43KD. The expected band size for MPPB is at 54KD.|Figure 2. IHC analysis using anti- PMPCB antibody (M11793). detected in paraffin-embedded section of human Ovarian Serous Carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- PMPCB antibody (M11793). detected in paraffin-embedded section of human adenocarcinoma of lung tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. ICC analysis using anti- PMPCB antibody (M11793). was detected in immersion fixed MCF7 cell. Cells were stained using the Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126) .and counterstained with DAPI (blue).|Figure 5. Flow cytometry analysis of JK cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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