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克隆性 ：Polyclonal
适应物种 ：human, mouse
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：WB, IHC, FCM, ELISA
规格 ：50μl/100μl/150μl
产品概况
| 货号 | A04492-3 |
|---|---|
| 产品名称 | Anti-FKBP1A/1B Antibody |
| 基因名 | FKBP1A/1B |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 15KD |
| 免疫原 | E.coli-derived human FKBP1A/1B recombinant protein (Position: M1-H88). |
| 内容 | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Peptidyl-prolyl cis-trans isomerase FKBP1A/1B is an enzyme that in humans is encoded by the FKBP1A/1B gene. The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. |
| 研究类别 | 1. Aylett, C. H. S., Sauer, E., Imseng, S., Boehringer, D., Hall, M. N., Ban, N., Maier, T. Architecture of human mTOR complex 1. Science 351: 48-52, 2016.2. DiLella, A. G., Hawkins, A., Craig, R. J., Schreiber, S. L., Griffin, C. A. Chromosomal band assignments of the genes encoding human FKBP12 and FKBP13. Biochem. Biophys. Res. Commun. 189: 819-823, 1992. 3. DiLella, A. G. Chromosomal assignment of the human immunophilin FKBP-12 gene. Biochem. Biophys. Res. Commun. 179: 1427-1433, 1991. |
| Uniprot ID | FKBP1A/1B: P62942/P68106 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| (ELISA)： | 1:100-1000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- FKBP1A/1B antibody (A04492-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: MOLT-4 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse kidney tissue lysates.
Use rabbit anti- FKBP1A/1B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FKBP1A/1B at approximately 15KD. The expected band size for FKBP1A/1B is at 12KD.|Figure 2. IHC analysis using anti- FKBP1A/1B antibody (A04492-3). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. Flow cytometry analysis of RT4 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 4. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: HepG2 whole cell lysates,
Lane 2: MOLT-4 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse kidney tissue lysates.
Use rabbit anti- FKBP1A/1B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FKBP1A/1B at approximately 15KD. The expected band size for FKBP1A/1B is at 12KD.|Figure 2. IHC analysis using anti- FKBP1A/1B antibody (A04492-3). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. Flow cytometry analysis of RT4 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 4. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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