Anti-Proteasome 20S beta 7/PSMB7 Antibody (monoclonal, 3H6C8)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：M08095-2

产品详情

产品概况

货号	M08095-2
产品名称	Anti-Proteasome 20S beta 7/PSMB7 Antibody (monoclonal, 3H6C8)
基因名	PSMB7
抗体来源	Mouse
克隆	Monoclonal(Clone:3H6C8)
抗体亚型	Mouse IgG2b
分子量	25KD
免疫原	E.coli-derived human Proteasome 20S beta 7/PSMB7 recombinant protein (Position: D17-S277).
内容	500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Proteasome subunit beta type-7 as known as 20S proteasome subunit beta-2 is a protein that in humans is encoded by the PSMB7 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.
研究类别	1. Coux, O., Tanaka, K., Goldberg, A. L. Structure and functions of the 20S and 26S proteasomes. Ann. Rev. Biochem. 65: 801-847, 1996. 2. Hisamatsu, H., Shimbara, N., Saito, Y., Kristensen, P., Hendil, K. B., Fujiwara, T., Takahashi, E., Tanahashi, N., Tamura, T., Ichihara, A., Tanaka, K. Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma. J. Exp. Med. 183: 1807-1816, 1996.
Uniprot ID	PSMB7: Q99436
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:500-2000
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- Proteasome 20S beta 7/PSMB7 antibody (M08095-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
Use mouse anti- Proteasome 20S beta 7/PSMB7 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Proteasome 20S beta 7/PSMB7 at approximately 25KD. The expected band size for Proteasome 20S beta 7/PSMB7 is at 30KD.|Figure 2. Flow cytometry analysis of Caco-2 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]