Anti-VWF Antibody (Clone#OTI2F2)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：MA00270

产品详情

产品概况

货号	MA00270
产品名称	Anti-VWF Antibody (Clone#OTI2F2)
基因名	VWF
抗体来源	Mouse
克隆	OTI2F2
抗体亚型	Mouse IgG2a
分子量	309.3KD
免疫原	Human recombinant protein fragment corresponding to amino acids 764-1263 of human VWF (NP_000543) produced in SF9 cell.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
研究类别	1. Sadler JE (1998). "Biochemistry and genetics of von Willebrand factor". Annu. Rev. Biochem. 67: 395–424. 2. Ginsburg, D., Handin, R. I., Bonthron, D. T., Donlon, T. A., Bruns, G. A. P., Latt, S. A., Orkin, S. H. Human von Willebrand factor (VWF): isolation of complementary DNA (cDNA) clones and chromosomal localization. Science 228: 1401-1406, 1985. 3. Sporn, L. A., Marder, V. J., Wagner, D. D. Von Willebrand factor released from Weibel-Palade bodies binds more avidly to extracellular matrix than that secreted constitutively. Blood 69: 1531-1534, 1987.
Uniprot ID	VWF: P04275

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:2000
Immunohistochemistry in paraffin section (IHC):	1:150

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VWF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00270)|Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWF. [/list_product_images]