Anti-CD45/PTPRC Antibody (Clone#OTI1H2)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：MA00555

产品详情

产品概况

货号	MA00555
产品名称	Anti-CD45/PTPRC Antibody (Clone#OTI1H2)
基因名	PTPRC
抗体来源	Mouse
克隆	OTI1H2
抗体亚型	Mouse IgG1
分子量	147.1KD
免疫原	Full length human recombinant protein of human PTPRC(NP_002829) produced in HEK293T cell.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
研究类别	1. Anderson, J.N. et al. (2004) FASEB J. 18:8.2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987.3. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
Uniprot ID	PTPRC: P06800

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:200~8000
Flow cytometry (FCM):	1:50

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Flow cytometric Analysis of living Jurkat cells, using anti-PTPRC antibody, (Red), compared to IgG isotype control, (green) and PBS, (blue).|Figure 2. HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human PTPRC produced in HEK293T cell (5 ug, right lane)were separated by SDS-PAGE and immunoblotted with anti-PTPRC.|Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).[/list_product_images]