Anti-GFI1 Antibody (Clone#OTI6E8)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal

适应物种：human, mouse, rat

保质期：发货周期：5-7天

库存：9999

供应商：博士德生物

应用范围：WB, IHC

规格：50μl/100μl/150μl

产品概况

货号	MA00888
产品名称	Anti-GFI1 Antibody (Clone#OTI6E8)
基因名	GFI1
抗体来源	Mouse
克隆	OTI6E8
抗体亚型	Mouse IgG1
分子量	45.1KD
免疫原	Human recombinant protein fragment corresponding to amino acids 1-180 of human GFI1(NP_005254) produced in E.coli.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
研究类别	1. Bell, D. W., Taguchi, T., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Gilks, C. B., Zweidler-McKay, P., Grimes, H. L., Tsichlis, P. N., Testa, J. R. Chromosomal localization of a gene, GFI1, encoding a novel zinc finger protein reveals a new syntenic region between man and rodents. Cytogenet. Cell Genet. 70: 263-267, 1995. 2. Duan, Z., Horwitz, M. Targets of the transcriptional repressor oncoprotein Gfi-1. Proc. Nat. Acad. Sci. 100: 5932-5937, 2003. Note: Erratum: Proc. Nat. Acad. Sci. 108: 16134 only, 2011. 3. Gilks, C. B., Bear, S. E., Grimes, H. L., Tsichlis, P. N. Progression of interleukin-2 (IL-2)-dependent rat T cell lymphoma lines to IL-2-independent growth following activation of a gene (Gfi1) encoding a novel zinc finger protein. Molec. Cell Biol. 13: 1759-1768, 1993.
Uniprot ID	GFI1: Q99684

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:2000
Immunohistochemistry in paraffin section (IHC):	1:150

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00888)|Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00888)|Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00888)|Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00888)|Figure 5. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00888)|Figure 6. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFI1(Cat# MA00888). [/list_product_images]