Anti-CD35 Antibody (Clone#OTI2A8)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal

适应物种：human

保质期：发货周期：5-7天

库存：9999

供应商：博士德生物

应用范围：WB, IHC

规格：50μl/100μl/150μl

产品概况

货号	MA01022
产品名称	Anti-CD35 Antibody (Clone#OTI2A8)
基因名	CR1
抗体来源	Mouse
克隆	OTI2A8
抗体亚型	Mouse IgG2b
免疫原	Human recombinant protein fragment corresponding to amino acids 1900-2039 of human CR1 (NP_000564) produced in E.coli.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]
研究类别	1. Jacobson AC, Weis JH (Sep 2008). "Comparative functional evolution of human and mouse CR1 and CR2". J. Immunol. 181 (5): 2953–9.2. Khera R, Das N (February 2009). "Complement Receptor 1: Disease associations and therapeutic implications". Molecular Immunology. 46 (5): 761–772. 3. Moulds JM, Nickells MW, Moulds JJ, Brown MC, Atkinson JP (May 1991). "The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera". J. Exp. Med. 173 (5): 1159–63.
Uniprot ID	CR1: P17927

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:2000
Immunohistochemistry in paraffin section (IHC):	1:500

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA01022) (1:500)|Figure 2. Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-CR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA01022) (1:500)|Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CR1 (Cat# MA01022)(1:2000).[/list_product_images]