Anti-SATB1 Antibody (Clone#OTI13D6)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal

适应物种：human, mouse, rat

保质期：发货周期：5-7天

库存：9999

供应商：博士德生物

应用范围：WB, IHC, IF

规格：50μl/100μl/150μl

产品概况

货号	MA01312
产品名称	Anti-SATB1 Antibody (Clone#OTI13D6)
基因名	SATB1
抗体来源	Mouse
克隆	OTI13D6
抗体亚型	Mouse IgG1
分子量	86KD
免疫原	Full-length protein expressed in 293T cell transfected with human SATB1 expression vector
内容	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	The protein binds to DNA at special AT-rich sequences at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA.
研究类别	1. Galande S, Purbey PK, Notani D, Kumar PP (Oct 2007). "The third dimension of gene regulation: organization of dynamic chromatin loopscape by SATB1". Current Opinion in Genetics & Development. 17 (5): 408–14.2. Pavan Kumar P, Purbey PK, Sinha CK, Notani D, Limaye A, Jayani RS, Galande S (Apr 2006). "Phosphorylation of SATB1, a global gene regulator, acts as a molecular switch regulating its transcriptional activity in vivo". Molecular Cell. 22 (2): 231–43.
Uniprot ID	SATB1: Q01826

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:2000
Immunohistochemistry in paraffin section (IHC):	1:50
Immunofluorescence (IF):	1:100

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.|Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 9. Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 10. Anti-SATB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1 .|Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01312, Dilution 1:50)|Figure 12. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1(Cat# MA01312). [/list_product_images]