Anti-IGF2BP2 Antibody (Clone#OTI4C4)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：MA02010

产品详情

产品概况

货号	MA02010
产品名称	Anti-IGF2BP2 Antibody (Clone#OTI4C4)
基因名	IGF2BP2
抗体来源	Mouse
克隆	OTI4C4
抗体亚型	Mouse IgG1
分子量	65.9KD
免疫原	Full length human recombinant protein of human IGF2BP2 (NP_006333) produced in HEK293T cell.
内容	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Uniprot ID	IGF2BP2: Q9Y6M1

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:1000~2000
Immunohistochemistry in paraffin section (IHC):	1:50
Immunofluorescence (IF):	1:100
Flow cytometry (FCM):	1:100

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.|Figure 2. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02010, Dilution 1:50)|Figure 3. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02010, Dilution 1:50)|Figure 4. Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02010, Dilution 1:50)|Figure 5. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.|Figure 6. Anti-IGF2BP2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2 .|Figure 7. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2. [/list_product_images]