Anti-F13A1 Antibody (Clone#OTI3F6)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal

适应物种：human, mouse, rat

保质期：发货周期：5-7天

库存：9999

供应商：博士德生物

应用范围：WB, IHC

规格：50μl/100μl/150μl

产品概况

货号	MA02047
产品名称	Anti-F13A1 Antibody (Clone#OTI3F6)
基因名	F13A1
抗体来源	Mouse
克隆	OTI3F6
抗体亚型	Mouse IgG1
分子量	79.2KD
免疫原	Full length human recombinant protein of human F13A1 (NP_000120) produced in HEK293T cell.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]
研究类别	1. AbdAlla, S., Lother, H., Langer, A., el Faramawy, Y., Quitterer, U. Factor XIIIA transglutaminase crosslinks AT1 receptor dimers of monocytes at the onset of atherosclerosis. Cell 119: 343-354, 2004. 2. Anwar, R., Langlois, S. The arg703-to-trp missense mutation in F13A1 is a de novo event. Brit. J. Haemat. 146: 118-120, 2009. 3. Ariens, R. A. S., Philippou, H., Nagaswami, C., Weisel, J. W., Lane, D. A., Grant, P. J. The factor XIII V34L polymorphism accelerates thrombin activation of factor XIII and affects cross-linked fibrin structure. Blood 96: 988-995, 2000.
Uniprot ID	F13A1: P00488

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:2000
Immunohistochemistry in paraffin section (IHC):	1:150

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-F13A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA02047)|Figure 2. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-F13A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA02047)|Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-F13A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA02047)|Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY F13A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-F13A1. [/list_product_images]