Anti-RANGAP1 Antibody (Clone#OTI1B4)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal

适应物种：human, mouse, rat, dog

保质期：发货周期：5-7天

库存：9999

供应商：博士德生物

应用范围：WB, IHC, IF, FCM

规格：50μl/100μl/150μl

产品概况

货号	MA02771
产品名称	Anti-RANGAP1 Antibody (Clone#OTI1B4)
基因名	RANGAP1
抗体来源	Mouse
克隆	OTI1B4
抗体亚型	Mouse IgG2a
分子量	63.4KD
免疫原	Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
内容	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]
研究类别	1. Bernier-Villamor, V., Sampson, D. A., Matunis, M. J., Lima, C. D. Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1. Cell 108: 345-356, 2002. 2. Bischoff, F. R., Klebe, C., Kretschmer, J., Wittinghofer, A., Ponstingl, H. RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Nat. Acad. Sci. 91: 2587-2591, 1994. 3. Bischoff, F. R., Krebber, H., Kempf, T., Hermes, I., Ponstingl, H. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Nat. Acad. Sci. 92: 1749-1753, 1995.
Uniprot ID	RANGAP1: P46060

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:1000~2000
Immunohistochemistry in paraffin section (IHC):	1:50
Immunofluorescence (IF):	1:100
Flow cytometry (FCM):	1:100

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02771, Dilution 1:50)|Figure 2. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02771, Dilution 1:50)|Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.|Figure 4. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RANGAP1 antibody, and then analyzed by flow cytometry.|Figure 5. Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).|Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02771, Dilution 1:50)|Figure 7. Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RANGAP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).|Figure 8. Anti-RANGAP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RANGAP1 .|Figure 9. Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).|Figure 10. Western blot analysis of extracts (10ug) from a mouse cell line by using anti-RANGAP1 monoclonal antibody (1:200).|Figure 11. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1(Cat# MA02771). [/list_product_images]