产品详情
文献和实验
相关推荐
克隆性 :Monoclonal
适应物种 :human, mouse, rat
保质期 :发货周期:5-7天
库存 :9999
供应商 :博士德生物
应用范围 :WB, IF
规格 :50μl/100μl/150μl
产品概况
货号 | MA04494 |
---|---|
产品名称 | Anti-VEGFB Antibody (Clone#OTI2D6) |
基因名 | VEGFB |
抗体来源 | Mouse |
克隆 | OTI2D6 |
抗体亚型 | Mouse IgG1 |
分子量 | 21.4KD |
免疫原 | Full length human recombinant protein of human VEGFB(NP_003368) produced in HEK293T cell. |
内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 | This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011] |
研究类别 | 1. Gerace, L, Cirenei, N., Cappelletti, M., Petraroli, R., Sebastiani, F., Marziliano, N. Assignment of the mouse Vegfb gene to mouse chromosome 19B by in situ hybridization. Cytogenet. Cell Genet. 95: 242-243, 2001. 2. Silvestre, J.-S., Tamarat, R., Ebrahimian, T. G., Le-Roux, A., Clergue, M., Emmanuel, F., Duriez, M., Schwartz, B., Branellec, D., Levy, B. I. Vascular endothelial growth factor-B promotes in vivo angiogenesis. Circ. Res. 93: 114-123, 2003. |
Uniprot ID | VEGFB: P49765 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot (WB): | 1:4000 |
Immunofluorescence (IF): | 1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Anti-VEGFB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGFB .|Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB. [/list_product_images]
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限:17年