Anti-Apc11 ANAPC11 Antibody (Clone#OTI1A8)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：MA08555

产品详情

产品概况

货号	MA08555
产品名称	Anti-Apc11 ANAPC11 Antibody (Clone#OTI1A8)
基因名	ANAPC11
抗体来源	Mouse
克隆	OTI1A8
抗体亚型	Mouse IgG1
分子量	20.5KD
免疫原	Full length human recombinant protein of human ANAPC11(NP_001002244) produced in HEK293T cell.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	Enables cullin family protein binding activity and ubiquitin-ubiquitin ligase activity. Contributes to ubiquitin-protein transferase activity. Involved in protein K11-linked ubiquitination. Located in nucleolus and nucleoplasm. Part of anaphase-promoting complex.?
研究类别	1. Gmachl, M., Gieffers, C., Podtelejnikov, A. V., Mann, M., Peters, J.-M. The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. Proc. Nat. Acad. Sci. 97: 8973-8978, 2000. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/10/2012. 3. Mansfeld, J., Collin, P., Collins, M. O., Choudhary, J. S., Pines, J. APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment. Nature Cell Biol. 13: 1234-1243, 2011.
Uniprot ID	ANAPC11: Q9NYG5

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:4000
Immunohistochemistry in paraffin section (IHC):	1:150
Immunofluorescence (IF):	1:100

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA08555)|Figure 2. Anti-ANAPC11 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC11 .|Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC11. [/list_product_images]