Anti-MRPS32 MRPL42 Antibody (Clone#OTI1A4)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：MA13167

产品详情

产品概况

货号	MA13167
产品名称	Anti-MRPS32 MRPL42 Antibody (Clone#OTI1A4)
基因名	MRPL42
抗体来源	Mouse
克隆	OTI1A4
抗体亚型	Mouse IgG1
分子量	16.5KD
免疫原	Full length human recombinant protein of human MRPL42(NP_054769) produced in E.coli.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011]
Uniprot ID	MRPL42: Q9Y6G3

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:2000
Immunohistochemistry in paraffin section (IHC):	1:150

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MRPL42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA13167) (1:150)|Figure 2. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MRPL42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA13167) (1:150)|Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPL42 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPL42 (Cat# MA13167)(1:2000). [/list_product_images]