产品详情
文献和实验
相关推荐
克隆性 ：Polyclonal
适应物种 ：human
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：WB, FCM(Intracellular), Direct ELISA
规格 ：50μl/100μl/150μl
产品概况
| 货号 | A14923-1 |
|---|---|
| 产品名称 | Anti-PLET1 Antibody |
| 基因名 | PLET1 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 28KD |
| 免疫原 | E.coli-derived human PLET1 recombinant protein (Position: D46-K166). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | PLET?1 (placenta ?expressed transcript 1), also known as antigen mAgK114, is a 23 kDa membrane protein that is probably GPI?anchored. It is recognized by the antibodies MTS20 and MTS24. Mature mouse PLET?1, which most likely includes amino acids (aa) 28?216 of 237 aa, shares less than 60% aa sequence identity with the most closely related rat or human protein. A potential 194 aa mouse splice variant diverges after aa 149 but still contains a hydrophobic sequence at the Cterminus. PLET?1 is a marker of early thymic progenitor cells and pancreatic duct epithelium. |
| 研究类别 | 1. Depreter, M. G. L., Blair, N. F., Gaskell, T. L., Nowell, C. S., Davern, K., Pagliocca, A., Stenhouse, F. H., Farley, A. M., Fraser, A., Vrana, J., Robertson, K., Morahan, G., Tomlinson, S. R., Blackburn, C. C. Identification of Plet-1 as a specific marker of early thymic epithelial progenitor cells. Proc. Nat. Acad. Sci. 105: 961-966, 2008. 2. Zhao, S.-H., Simmons, D. G., Cross, J. C., Scheetz, T. E., Casavant, T. L., Soares, M. B., Tuggle, C. K. PLET1 (C11orf34), a highly expressed and processed novel gene in pig and mouse placenta, is transcribed but poorly spliced in human. Genomics 84: 114-125, 2004. |
| Uniprot ID | PLET1: Q6UQ28 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti-PLET1 antibody (A14923-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLET1 antigen affinity purified polyclonal antibody (A14923-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PLET1 at approximately 28 kDa. The expected band size for PLET1 is at 23,25 kDa.|Figure 2. Flow Cytometry analysis of SiHa cells using anti-PLET1 antibody (A14923-1).
Overlay histogram showing SiHa cells stained with A14923-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLET1 Antibody (A14923-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLET1 antigen affinity purified polyclonal antibody (A14923-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PLET1 at approximately 28 kDa. The expected band size for PLET1 is at 23,25 kDa.|Figure 2. Flow Cytometry analysis of SiHa cells using anti-PLET1 antibody (A14923-1).
Overlay histogram showing SiHa cells stained with A14923-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLET1 Antibody (A14923-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
