Anti-DAI/ZBP1 Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：human

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, ICC/IF, FCM, ELISA

规格：50μl/100μl/150μl

产品概况

货号	A04739-2
产品名称	Anti-DAI/ZBP1 Antibody
基因名	ZBP1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	63KD
免疫原	E.coli-derived human DAI/ZBP1 recombinant protein (Position: A2-E421).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Z-DNA-binding protein 1, also known as DNA-dependent activator of IFN-regulatory factors (DAI) and DLM-1, is a protein that in humans is encoded by the ZBP1 gene. This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.?
研究类别	1. Fu, Y., Comella, N., Tognazzi, K., Brown, L. F., Dvorak, H. F., Kocher, O. Cloning of DLM-1, a novel gene that is up-regulated in activated macrophages, using RNA differential display. Gene 240: 157-163, 1999. 2. Jiao, H., Wachsmuth, L., Kumari, S., Schwarzer, R., Lin, J., Eren, R. O., Fisher, A., Lane, R., Young, G. R., Kassiotis, G., Kaiser, W. J., Pasparakis, M. Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation. Nature 580: 391-395, 2020. Note: Erratum: Nature 580: E10, 2020. Electronic Article. 3. Rothenburg, S., Schwartz, T., Koch-Nolte, F., Haag, F. Complex regulation of the human gene for the Z-DNA binding protein DLM-1. Nucleic Acids Res. 30: 993-1000, 2002.
Uniprot ID	ZBP1: Q9H171
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunocytochemistry/Immunofluorescence (ICC/IF):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(ELISA)：	1:100-1000

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- ZBP1 antibody (A04739-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human HELA whole cell lysates,Lane 3: human K562 whole cell lysates.Lane 4: human CACO-2 whole cell lysates.Use rabbit anti- ZBP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ZBP1 at approximately 63KD. The expected band size for ZBP1 is at 63KD.|Figure 2. ICC analysis using anti- ZBP1 antibody (A04739-2). was detected in immersion fixed HELA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of U87 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]