Anti-IL-17F Antibody(monoclonal, AFIC-9)

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：M02062

产品详情

产品概况

货号	M02062
产品名称	Anti- IL-17F Antibody(monoclonal， AFIC-9)
基因名	Il17f
抗体来源	Mouse
克隆	Monoclonal(Clone:AFIC-9)
抗体亚型	Mouse IgG1, κ
免疫原	Mouse IL-17F
内容	200 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	200 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and hasbeen shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
Uniprot ID	Il17f: Q7TNI7
推荐配套的二抗和检测试剂	Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. IHC analysis of IL-17F using anti- IL-17F antibody (M02062).
IL-17F was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti- IL-17F Antibody (M02062) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]