Anti-CD45/PTPRC Antibody (monoclonal, 2H3)价格

克隆性：Monoclonal

适应物种：human

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC, IF, FCM

规格：50μl/100μl/150μl

产品概况

货号	M00555-5
产品名称	Anti-CD45/PTPRC Antibody (monoclonal, 2H3)
基因名	PTPRC
抗体来源	Mouse
克隆	Monoclonal(Clone:2H3)
抗体亚型	Mouse IgG2b
分子量	180-250KD
免疫原	A synthetic peptide corresponding to a sequence at the C-terminus of human CD45(1214-1254aa EQYQFLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVDKVK), different from the related mouse sequence by eight amino acids, and from the related rat sequence by ten amino acids.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. It is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. In addition, CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
研究类别	1. Anderson, J.N. et al. (2004) FASEB J. 18:8.2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987.3. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
Uniprot ID	PTPRC: P08575
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Immunofluorescence (IF):	1:50-400
Flow cytometry (FCM):	1-3μg/1x10⁶ cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of anti- CD45/PTPRC Antibody (M00555-5). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat tissue lysates,
Lane 2: Raji tissue lysates.
Use mouse anti-CD45/PTPRC 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD45/PTPRC at approximately 147KD. The expected band size for CD45/PTPRC is at 147KD.|Figure 2.IF analysis using anti- CD45/PTPRC Antibody (M00555-5).detected in paraffin-embedded section of human tonsli tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).|Figure 3. Flow cytometry analysis of Raji cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]