产品详情
文献和实验
相关推荐
克隆性 ：Polyclonal
适应物种 ：human
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：WB, IHC, ICC/IF, FCM, ELISA
规格 ：50μl/100μl/150μl
产品概况
| 货号 | A00705-1 |
|---|---|
| 产品名称 | Anti-SSB Antibody |
| 基因名 | SSB |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 47KD |
| 免疫原 | E.coli-derived human SSB recombinant protein (Position: D6-L283). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Sj?gren syndrome type B antigen (SS-B) also known as Lupus La protein is a protein that in humans is encoded by the SSB gene. The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. |
| 研究类别 | 1. Bini, P., Chu, J.-L., Okolo, C., Elkon, K. Analysis of autoantibodies to recombinant La (SS-B) peptides in systemic lupus erythematosus and primary Sjogren's syndrome. J. Clin. Invest. 85: 325-333, 1990. 2. Chambers, J. C., Kenan, D., Martin, B. J., Keene, J. D. Genomic structure and amino acid sequence domains of the human La autoantigen. J. Biol. Chem. 263: 18043-18051, 1988. 3. Grolz, D., Bachmann, M. The nuclear autoantigen La/SS-associated antigen B: one gene, three functional mRNAs. Biochem. J. 323: 151-158, 1997. |
| Uniprot ID | SSB: P05455 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti- SSB Antibody (A00705-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: K562 whole cell lysates,
Lane 2: HEL whole cell lysates,
Lane 3: HL-60 whole cell lysates.
Use rabbit anti- SSB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SSB at approximately 47KD. The expected band size for SSB is at 47KD.|Figure 2. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human Laryngeal carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human Hashimoto thyroiditis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.| Figure 6. ICC analysis using anti- SSB antibody (A00705-1). was detected in immersion fixed CACO-2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: K562 whole cell lysates,
Lane 2: HEL whole cell lysates,
Lane 3: HL-60 whole cell lysates.
Use rabbit anti- SSB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SSB at approximately 47KD. The expected band size for SSB is at 47KD.|Figure 2. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human Laryngeal carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human Hashimoto thyroiditis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SSB Antibody (A00705-1). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.| Figure 6. ICC analysis using anti- SSB antibody (A00705-1). was detected in immersion fixed CACO-2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
