Anti-NRP1 Antibody (monoclonal, 8G6G7)价格_品牌:BOSTER-丁香通官网

克隆性：Monoclonal

适应物种：human, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, ICC/IF, FCM

规格：50μl/100μl/150μl

产品概况

货号	M01324-2
产品名称	Anti-NRP1 Antibody (monoclonal, 8G6G7)
基因名	NRP1
抗体来源	Mouse
克隆	Monoclonal(Clone:8G6G7)
抗体亚型	Mouse IgG2a
分子量	120KD
免疫原	E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
研究类别	1. Gagnon, M. L., Bielenberg, D. R., Gechtman, Z., Miao, H.-Q., Takashima, S., Soker, S., Klagsbrun, M. Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor: in vivo expression and antitumor activity. Proc. Nat. Acad. Sci. 97: 2573-2578, 2000.2. Gu, C., Rodriguez, E. R., Riemert, D. V., Shu, T., Fritzsch, B., Richards, L. J., Kolodkin, A. L., Ginty, D. D. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev. Cell 5: 45-57, 2003.
Uniprot ID	NRP1: O14786
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunocytochemistry/Immunofluorescence(ICC/IF):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

点击图片放大

[list_product_images]Figure 1. Western blot analysis of anti- NRP1 Antibody (M01324-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: MCF-7 whole cell lysates,
Lane 5: rat stomach tissue lysates,
Use mouse anti- NRP1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for NRP1 at approximately 120KD. The expected band size for NRP1 is at 103KD.|Figure 2.ICC analysis using anti-NRP1 Antibody (M01324-2). was detected in immersion fixed A549 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) and counterstained with DAPI (blue).|Figure 3. Flow cytometry analysis of U-87MG cell (1x106) DyLight 488 conjugated goat anti- mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]