Anti-PSMA1 Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：human, mouse, rat

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, IHC

规格：50μl/100μl/150μl

产品概况

货号	PB10087
产品名称	Anti-PSMA1 Antibody
基因名	PSMA1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	30KD
免疫原	A synthetic peptide corresponding to a sequence at the C-terminus of human PSMA1 (159-204aa MSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLP AEQD), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Proteasome subunit alpha type-1 is a protein that in humans is encoded by the PSMA1 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
研究类别	1. Groll M, Ditzel L, L we J, Stock D, Bochtler M, Bartunik HD, Huber R (Apr 1997). "Structure of 20S proteasome from yeast at 2.4 A resolution". Nature 386 (6624): 463–71.2. Tomko RJ, Hochstrasser M (2013). "Molecular architecture and assembly of the eukaryotic proteasome".Annual Review of Biochemistry 82: 415–45.
Uniprot ID	PSMA1: P25786
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Western blot analysis of PSMA1 expression in rat testis extract (lane 1), mouse spleen extract (lane 2) and HEPG2 whole cell lysates (lane 3). PSMA1 at 29KD was detected using rabbit anti- PSMA1 Antigen Affinity purified polyclonal antibody (Catalog # PB10087) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|PSMA1 was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- PSMA1 Antigen Affinity purified polyclonal antibody (Catalog # PB10087) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).[/list_product_images]