Rat CCR7受体稳定表达细胞株

ScreeNingBio CHO-K1 Rat CCR7 Gi Cell Line
Background
CHO-K1 Rat CCR7 Gi cell line overexpress rat CCR7 and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.
Product Information
Catalog Number:           SNB-G-0058C
Lot Number:                 See vial
Contents:                     2 vials, 1 x 10⁶ cells per vial
Target GPCR:                C-C motif chemokine receptor 7 (CCR7)
HGNC Symbol:             CCR7
Coupling:                     Gi
Accession No.:             NM_199489
GPCR Species:                Rat
Host Cell:                    CHO-K1
Culture Medium:         F12 + 10% FBS
Antibiotics:                 200 μg/mL Hygromycin B
Storage:                      Store in vapor phase of liquid nitrogen upon receiving
Functional Assay Data
In a 384-well plate, cells were stimulated with agonist compounds using the assay conditions described below. Following stimulation, signal was detected using the Cisbio HTRF cAMP assay detection kit according to the recommended protocol.

Agonist EC50 was 0.72 nM and signal window 2.46 fold.

Passage Stability

This cell line has been confirmed to be stable through 10 passages (4 weeks) with no significant drop in assay window or change in EC50 of control compound.

 Price Information
￥79,999 for 2 vial of frozen cell
￥7,999 for one 96 well compound testing (4 compounds, 10 point, ½ log dilution in duplicate)
￥11,999 for one 384 well compound testing (16 compounds, 10 point, ½ log dilution in duplicate)
 For additional information, please visit www.screeningbio.com or info@screeningbio.com
上海格宁生物科技有限公司

上海格宁生物科技有限公司成立于2020年，以人类健康为使命，从事新药研发领域内的技术开发、咨询、转让和技术服务。公司主要科研人员均有多年新药开发领域工作经验，同时实验室拥有完善的细胞培养室、液体工作站、酶标仪等设备以支持相关重组细胞系开发和细胞生化水平的药物筛选服务。格宁生物已开发1000种以上各类重组功能细胞系，靶点涉及G蛋白偶联受体、离子通道、核受体、信号通路等，构建广泛的药物筛选平台，助力合作伙伴的科研进程。