人冠状病毒 (HCoV-229E) Nucleocapsid 表达质粒,C-HA

英文名 ：Human coronavirus 229E (HCoV-229E) Nucleocapsid / NP Gene ORF cDNA clone expression plasmid, C-HA tag

库存 ：大量

供应商 ：北京义翘神州科技股份有限公司

规格 ：1 Unit

Human coronavirus 229E (HCoV-229E) Nucleocapsid / NP Gene ORF cDNA clone expression plasmid, C-HA tag: 产品信息
基因
种属    HCoV-229E
NCBI 参考序列号    NC_002645.1(25686-26855nt)
基因长度    1212 bp
序列特征    Identical with the Gene Bank Ref. ID sequence.
产品特征    Full length Clone DNA of Human coronavirus(HCoV-229E) Nucleoprotein / NP.

质粒
启动子    Enhanced CMV mammalian cell promoter
载体    pCMV3-C-HA
限制性酶切位点    KpnI(two restriction sites) + XbaI(6kb+0.13kb+1.09kb)
标签序列    HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物    T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控    The plasmid is confirmed by full-length sequencing.

筛选
细菌筛选抗性    Kanamycin
细胞筛选抗性    Hygromycin
应用    Stable or Transient mammalian expression

储存 & 运输
运输方式    Each tube contains lyophilized plasmid.
储存条件    The lyophilized plasmid can be stored at ambient temperature for three months.

Nucleoprotein/NP cDNA ORF 核苷酸序列及氨基酸序列信息
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Nucleoprotein/NP 背景信息
Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.