BD Pharmingen 556547 Annexin V-FITC 细胞凋亡检测试剂盒

库存 ：99

供应商 ：北京诺博莱德科技有限公司

所属品牌：BD
产品名称：BD Pharmingen 556547 Annexin V-FITC 细胞凋亡检测试剂盒
产品货号：556547
产品规格：100T
产品单位：盒
产品说明：
商品详情：
 
Description
Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation.
 
Components

Part   No.	Description	Size
51-65874X	FITC   Annexin V	0.5   mL (1 ea)
51-66211E	Propidium   Iodide Staining Solution	2   mL (1 ea)
51-66121E	10X   Annexin V Binding Buffer	50   mL (1 ea)

 
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
 
PRODUCT NOTICES
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.