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库存 ：8 Rxns、48 Rxns
英文名 ：NEXTflex™ Rapid Directional RNA-Seq Kit
保质期 ：>1年
供应商 ：蓝景科信
保存条件 ：-20℃/2~8℃
NEXTflex™ Rapid Directional RNA-Seq Kit 为mRNA 或去除rRNA 总RNA的单端、双端和多重链特异性文库构建提供一个简单、灵活的解决方案。这个试剂盒可以用于获取RNA转录中DNA模板链的信息，准确率大于99%。与普通转录组测序相比，它更能准确地统计转录本的数量和确定基因的结构，同时可以发现更多的反义转录本，目前被广泛地应用于研究基因结构和基因表达调控等领域范围，并能够减少每个样本的测序费用。
NEXTflex™ Rapid Directional mRNA-Seq Kit 套装
为了简化mRNA 测序，PerkinElmer公司现在提供一个套装，包括NEXTflex Rapid Directional RNA-Seq library 建库试剂，NEXTflex RNA-Seq Barcodes 和NEXTflex Poly(A) Beads 。这个试剂盒有两个不同的版。每个试剂盒包含48次的NEXTflex Rapid Directional RNA-Seq Kit，NEXTflex Poly(A) Beads (48 rxns )和24种独立NEXTflex RNA-Seq Barcodes。
产品特点：
- 快速的建库流程，节省人工操作时间
- 合理化的实验方案，减少样本损失
- 基于dUTP的方法
- 提供精确的mRNA 链定向测量(>99%)
- 稳定的、可靠的实验流程
- 起始量1-100 ng mRNA或者去除rRNA 的总RNA
- 多达96种barcodes 运用于多重测序
- 匹配PE Sciclone的NGS和NGSx自动化建库系统
- 经Illumina 测序平台验证
- 可用于单端或者双端转录组测序
多重测序
NEXTflex Rapid Directional RNA-Seq Kit与NEXTflex™ RNA-Seq Barcodes 或者NEXTflex-96™ RNA-Seq Barcodes一起用于多重测序。这个多达96种独立接头的高通量试剂盒适用于Illumina RNA 测序平台。
产品列表：
| 货号 | 产品名称 | 规格 |
| NOVA-5138-07 | NEXTflex™ Rapid Directional RNA-Seq Kit |
8 RXNS |
| NOVA-5138-08 | NEXTflex™ Rapid Directional RNA-Seq Kit |
48 RXNS |
| NOVA-5138-10 | NEXTflex™ W/ RNA-SEQ BARCODES 1 - 24 & POLY(A) BEADS |
48 RXNS |
| NOVA-5138-11 | NEXTflex™ W/ RNA-SEQ BARCODES 25 - 48 & POLY(A) BEADS | 48 RXNS |
Figure 1.与竞争者N 文库产量的比较
1) 插入片段范围为200-400bp（Agilent Bioanalyzer）（Figure 5A）
2) Figure 1B 代表Comp N的文库量，Figure 5C 代表NEXTflex 1:5 稀释的文库量，NEXTflex的文库浓度是Competitor N的四倍
rRNA and Other Non-Coding RNA
Figure 2. rRNA Mapping
RNA建库过程有少量的rRNA污染，但是NEXTflex Rapid Directional RNA-Seq Kit与Competitor N’s kit相比，rRNA 的读取率低，文库量高，有用的测序reads所占比例高
Read Mapping
Table 1. Unique Mapping Rate and Number of Unique Reads.
| Kit Name | Unique Mapped | Unique Mapped Reads (millions) |
| NEXTflex™ Rapid Directional RNA-Seq Kit | 53.49% ± 1.16% | 1.604 ± 35 |
| Competitor N Directional RNA-Seq Kit | 44.08% ± 3.95% | 1.321 ± 118 |
Figure 3. Reads Mapping Metrics
NEXTflex Rapid Directional RNA-Seq Kit比较结果：
1) 更高的唯一mapped reads百分比
2) 较低的重复率
3) 检测出更多的转录本
KIT CONTENTS
- NEXTFLEX® RNA Fragmentation Buffer
- NEXTFLEX® First Strand Synthesis Primer
- NEXTFLEX® Directional First Strand Synthesis Buffer Mix
- NEXTFLEX® Rapid Reverse Transcriptase
- NEXTFLEX® Directional Second Strand Synthesis Mix
- NEXTFLEX® Adenylation Mix
- NEXTFLEX® Ligation Mix
- NEXTFLEX® RNA-Seq Barcode Adapter 1 (0.6 µM)
- NEXTFLEX® Uracil DNA Glycosylase
- NEXTFLEX® Primer Mix (12.5 µM)
- NEXTFLEX® PCR Master Mix
- Nuclease-free Water
- Resuspension Buffer
REQUIRED MATERIALS NOT PROVIDED
- 10 ng – 1 µg total RNA for enrichment by NEXTFLEX® Poly(A) Beads 2.0 or ~ 1 ng – 100 ng isolated mRNA or rRNA-depleted RNA
- DynaMag™-2 Magnet (Life Technologies Cat # 123-21D)
- Commercial kits for rRNA depletion (optional, if not using poly(A) enrichment)
- 100% Ethanol (stored at room temperature)
- 80% Ethanol ( freshly prepared )
- 2, 10, 20, 200 and 1000 µL pipettes
- RNase-free pipette tips
- Nuclease-free 1.5 mL microcentrifuge tubes
- Thin wall nuclease-free 0.5 mL microcentrifuge tubes
- 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
- Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
- Agencourt AMPure XP 60 mL (Beckman Coulter Genomics, Cat # A63881)
- Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar for post PCR cleanup
- Microcentrifuge
- Thermocycler
- Heat block
- Vortex
蓝景科信河北生物科技有限公司
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入驻年限：2年
