文献支持NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers

库存 ：8 Rxns、48 Rxns、96 Rxns、192 Rxns、384 Rxns

英文名 ：NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers

保质期 ：>1年

供应商 ：蓝景科信

保存条件 ：-20℃/2~8℃

NEXTflex™ DNA Sequencing Kit - Ion Torrent Compatible 适用于 genomic DNA-Seq或 ChIP-Seq高通量测序建库，兼容于Ion S5, Ion S5 XL，Ion Torrent PGM 和 Ion Proton 测序平台。NEXTflex DNA Sequencing Kit 采用了“Enhanced Adapter Ligation Technology ”，得到更长的、种类更多的测序reads。这款 DNA-Seq library prep kit 通过使用酶预混液、凝胶纯化来简化文库制备流程。此外，可在96孔板中进行建库反应，可更好的实现建库自动化。

 

兼容于Ion S5, Ion PGM 和 Ion Proton 平台的多重DNA建库

NEXTflex™ DNA Barcodes - Ion Torrent Compatible中接头包含index序列，改进了多重建库实验流程，样本起始量也可进行灵活的选择。可同时进行多达64种样本建库。NEXTflex DNA Barcodes分别包含8, 16,32, and64重独特接头。此建库方案可有效的减少实验耗时，获得稳定的实验数据。

 

产品特点：

• 较高的接头连接效率，产出大量不同的reads
• 灵活的接头方案供选择—试剂盒包含8、16、32和64重独特barcodes
• 简化实验流程，实现自动化建库
• 适用于 Ion Torrent 平台的ChIP-seq 和DNA-seq 建库
• 起始量为10 ng，100 ng或 1 μg 基因组DNA

 

产品列表：

货号	产品名称	规格
NOVA-4001-01	NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers	8 RXNS
NOVA-4001-02	NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers	48 RXNS
NOVA-401001	NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 8	48 RXNS
NOVA-401002	NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 16	96 RXNS
NOVA-401003	NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 32	192 RXNS
NOVA-401004	NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 64	384 RXNS

 

NEXTflex™ DNA-Seq Kit Protocol 

KIT CONTENTS

• NEXTFLEX® End Repair Buffer Mix
• NEXTFLEX® End Repair Enzyme Mix
• NEXTFLEX® Ligation Mix
• NEXTFLEX® Adapter Mix (6.25 µM)
• NEXTFLEX® Primer Mix (12.5 µM)
• NEXTFLEX® 5X PCR Master Mix
• 6X Loading Dye
• MW Ladder Ready-to-Load 100 bp
• Column Elution Buffer
• Nuclease-free Water
• Resuspension Buffer
• DNA Binding Buffer
• 5X DNA Wash Buffer
• Clean-Up Spin Columns

REQUIRED  MATERIALS NOT PROVIDED

• 10 ng, 100 ng or 1 µg of fragmented genomic DNA in up to 40 µL nuclease-free water.
• NEXTFLEX® DNA Barcodes Ion Torrent Compatible – 8 / 16 / 32 / 64 (Cat # 4010-01, 4010-02, 4010-03, 4010-04)
• Ethanol 100% (room temperature)
• Ethanol 80% (room temperature)
• 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
• 96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
• Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
• Agencourt AMPure XP 5 mL (Beckman Coulter Genomics, Cat # A63880)
• Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
• Heat block
• Thermocycler
• 2, 10, 20, 200 and 1000 mL pipettes / multichannel pipettes
• Nuclease-free barrier pipette tips
• Microcentrifuge
• 1.5 mL nuclease-free microcentrifuge tubes
• Low melt agarose such as Low Gelling Temperature Agarose with a melt point of 65ºC (Boston Bioproducts, Cat # P-730) / or / E-gel system
• 1X TAE buffer
• Clean razor or scalpel
• SYBR Gold (Invitrogen, Cat # S11494)
• UV transilluminator or gel documentation instrument
• Gel electrophoresis apparatus
• Electrophoresis power supply
• Vortex

 

NEXTflex DNA Sequencing Kit for Ion Torrent Sequencing部分引用文献

Appenzeller, S. et al. (2015) Immunoglobulin rearrangement analysis from multiple lesions in the same patient using Next Generation Sequencing. Histopathology. doi: 10.1111/his.12714.