库存 ：24rxn
英文名 ：riboPOOL 24 reactions probes (Pan-Pro)
保质期 ：1年
供应商 ：蓝景科信
保存条件 ：-20℃
原核通用型riboPOOLs核糖体RNA去除
原核通用型 riboPOOL设计依据：
基于76个代表性物种的rRNAs 序列设计的微生物通用型 riboPOOL，适合图中所示的类群(内圈，粗体)和亚群(外圈)所涵盖的细菌和古细菌。
*如果您感兴趣的物种不属于这些类群或亚群，请咨询我们的定制型riboPOOLs产品。
**译注:暂无中文译名
原核通用型 riboPOOL已验证物种（RNA-Seq）
测试结果来自不同的实验室，rRNA去除效率从~50-98%不等，在大多数物种中rRNA去除效率> 70% 。去除效率可能因样品类型和实验条件的不同而不同。高质量的RNA样本中效率最高。推荐的起始RNA投放量为100 ng - 5 μg。
| 已检测的物种 |
去除效率验证 |
| 大肠杆菌 |
√ |
| 鼠伤寒 沙门氏菌 |
√ |
| 霍乱弧 菌 |
√ |
| 金黄色 葡萄球菌 |
√ |
| 凯伍热厌氧菌 |
√ |
| 鲍氏不动杆菌 |
√ |
| 恶臭假单胞菌 |
√ |
| 热醋穆尔氏菌 |
√ |
| 紫色杆菌 |
√ |
| 具核梭杆菌 |
√ |
| 幽门螺杆 菌 |
√ |
| 空肠弯曲杆菌 |
√ |
| 艰难梭菌 |
√ |
riboPOOLs应用方向：
除了rRNA外，在测序数据中发现的丰富的组织特异性mRNA（如珠蛋白）或其他丰富的RNA的去除也可以用riboPOOLs来进行。
RNA-seq是一种高度准确且灵敏的转录组追踪工具。然而，一些丰度很高的RNA种类却影响了NGS文库制备，浪费了宝贵的测序资源。在提取的总RNA中，核糖体RNA（rRNA）占比最大，约90%-95%，mRNA占2-3%，还有2-3%是非编码RNA（lncRNA、tRNA、microRNA等）。所以，在RNA测序的过程当中，首先要解决的问题，是如何去除核糖体RNA。riboPOOLs能够高效去除与研究无关的RNA，让研究人员更高效地利用测序资源。
核糖体分析涉及核糖体结合RNA的测序，以评估翻译组。在RNA-Seq之前，需将受核糖体保护的RNA片段分离出来，并进行rRNA去除。由于这些片段的范围为17-35个核苷酸，rRNA的去除效率预计将低于典型的RNA样本。人类riboPOOL已被应用，且rRNA去除效率在50-80%之间。为了进一步增强rRNA的去除能力，在rRNA去除后，还可以采用定制化riboPOOL进一步去除残余rRNA序列。
- 宏转录组(Metatranscriptomics)测序分析
Pan-Prokaryote riboPOOL被设计用来从所有主要门的广谱微生物中去除rRNA，使其成为一种通用的微生物rRNA去除工具。它可用于含有已知和未知微生物（如土壤、污泥、病原体感染组织）的复杂环境混合物或微生物组样本的rRNA去除。
为了从含有多个物种的样本中去除rRNA，或为便于在包含多个物种的样本中使用一种试剂盒，可以使用组合riboPOOLs。这些可由客户指定以一定比例进行组合，至多可实现四种现成riboPOOLs的混合物。对高质量RNA样本的内部测试显示，组合riboPOOLs（人类/小鼠/泛原核生物）从人类、小鼠和大肠杆菌RNA中去除rRNA的表现与物种特异性 riboPOOLs效果类似。然而，siTOOLs不能确保组合riboPOOLs在所有样品类型中具有相似的去除效率，因为去除效率也高度依赖于样品的质量。
该产品被引用文献
Hadjeras et al. (2023) Unraveling the small proteome of the plant symbiont Sinorhizobium meliloti by ribosome profiling and proteogenomics microLife
Li et al. (2023) Differential regulation of mRNA stability modulates transcriptional memory and facilitates environmental adaptation Nature Communications
Hadjeras et al. (2023) Revealing the small proteome of Haloferax volcanii by combining ribosome profiling and small-protein optimized mass spectrometry microLife
Matiasek et al. (2023) Mystery of fatal ‘staggering disease’ unravelled: novel rustrela virus causes severe meningoencephalomyelitis in domestic cats Nature communications
Steiner et al. (2023) Central Role of Sibling Small RNAs NgncR_162 and NgncR_163 in Main Metabolic Pathways of Neisseria gonorrhoeae mBio
Sahonero-Canavesi et al. (2022) Disentangling the lipid divide: Identification of key enzymes for the biosynthesis of membrane-spanning and ether lipids in Bacteria Science Advances
Zhang et al. (2022) A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis Plant Methods
Palomar-Siles et al. (2022) Translational readthrough of nonsense mutant TP53 by mRNA incorporation of 5-Fluorouridine Cell Death & Disease
Snieckute et al. (2022) Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response Cell Metabolism
Kim et al. (2022) Characterization of an Entner–Doudoroff pathway-activated Escherichia coli Biotechnology for Biofuels and Bioproducts
Cannon et al. (2022) Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 PLOS Pathogens
Fernández-Justel et al. (2022) Histone H1 regulates non-coding RNA turnover on chromatin in a m6A-dependent manner Cell Reports
Meindl et al. (2022) A rapid protocol for ribosome profiling of low input samples bioRxiv also cites siFractor
Li et al. (2022) Sensing of individual stalled 80S ribosomes by Fap1 for nonfunctional rRNA turnover Molecular Cell
Fritz et al. (2022) Transcriptomic fingerprint of bacterial infection in lower extremity ulcers APMIS
Chen et al. (2022) Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seq STAR Protocols
Jia et al. (2022) An atlas of plant full-length RNA reveals tissue-specific and monocots–dicots conserved regulation of poly(A) tail length Nature Plants
Alarcón-Schumacher et al. (2022) Isolation of a virus causing a chronic infection in the archaeal model organism Haloferax volcanii reveals antiviral activities of a provirus PNAS
Waditee‑Sirisattha et al. (2022) Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418 Scientific Reports
Jung et al. (2022) Opportunities and challenges to profile mRNA modifications in Escherichia coli ChemBioChem
Goar et al. (2022) Molecular Connectivity between Extracytoplasmic Sigma Factors and PhoP Accounts for Coupled Mycobacterial Stress Response Journal of Bacteriology
Pastor et al. (2022) Comparative Analysis of rRNA Removal Methods for RNA-Seq Differential Expression in Halophilic Archaea Biomolecules
Wahl et al. (2022) Comparison of rRNA depletion methods for efficient bacterial mRNA sequencing Scientific Reports
Wittekind et al. (2022) The novel protein ScrA acts through the SaeRS two component system to regulate virulence gene expression in Staphylococcus aureus Molecular Microbiology
Fujii et al. (2022) Ribotype-dependent growth inhibition and promotion by erythritol in Cutibacterium acnes Journal of Cosmetic Dermatology
Puiggené, Ò. et al. (2022) Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1 Scientific Reports
M. A. Lawlo et al. (2021) A transposon expression burst accompanies the activation of Y-chromosome fertility genes during Drosophila spermatogenesis Nature Communications
Felix Grünberger et al. (2021) Nanopore sequencing of RNA and cDNA molecules in Escherichia coli RNA
Martin Gélinas et al. (2021) The de novo Purine Biosynthesis Pathway Is the Only Commonly Regulated Cellular Pathway during Biofilm Formation in TSBBased Medium in Staphylococcus aureus and Enterococcus faecalis Microbiology Spectrum
Weipeng Mo et al. (2021) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing Genome Biology
Dimitar Plamenov Petrov et al. (2021) Quantitative profiling and dynamics of mRNA modifications in Escherichia coli
Bhat et al. (2021) Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome Science
Mio Takeuchi, Hideyoshi Yoshioka (2021) Acetate excretion by a methanotroph, Methylocaldum marinum S8, under aerobic conditions Bioscience, Biotechnology, and Biochemistry
Blaze et al (2021) Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior Nature Communications
Y. Long (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing Nature Protocols
Neil Fleck & Christoph Grundner (2021) A Cas12a-based CRISPR interference system for multigene regulation in mycobacteria JBC
J. Andersen et al (2021) Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms npj Biofilms and Microbiomes
La Rosa et al (2021) Compensatory evolution of Pseudomonas aeruginosa’s slow growth phenotype suggests mechanisms of adaptation in cystic fibrosis Nature Communications
Fuchs et al (2021) Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach PLOS Genetics
Martin Benda (2020) PhD dissertation A possible functional link between RNA degradation and transcription in Bacillus subtilis
Ryan. D et. al (2020) A high-resolution transcriptome map identifies small RNA regulation of metabolism in the gutmicrobe Bacteroides thetaiotaomicron. Nature Communications
Kim, I et. al (2019) Efficient depletion of ribosomal RNA for RNA sequencing in planarians. BMC Genomics
Galmozzi, C. V. et. al (2019) Selective ribosome profiling to study interactions of translating ribosomes in yeast. Nature Protocols
