产品详情
文献和实验
相关推荐
库存 ：99
英文名 ：TheWell BM01 Cyto3D® Live-Dead Assay Kit
供应商 ：北京云肽生物科技有限公司
TheWell BM01 Cyto3D® Live-Dead Assay Kit
The Well Bioscience是一家位于美国新泽西州的高科技创新生物技术公司。成立于2017年，专注于开发更易用的三维细胞培养的解决方案。可自定义的生物功能性和水凝胶生物力学的*组合使其成为三维细胞培养或再生医学的良好基质。
TheWell公司专注于3D细胞培养产品，为细胞再创体内生长环境。VitrogelTM3D 细胞培养水凝胶为无动物源性多糖水凝胶体系，模仿天然细胞外基质环境，可用于悬浮细胞和贴壁细胞的培养，为体内和体外研究提供共通的操作平台。
我们北京云肽生物科技有限公司作为TheWell公司的特约经销商为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。
TheWell BM01 Cyto3D® Live-Dead Assay Kit，产品简介：
产品品牌：TheWell
产品名称：Cyto3D® Live-Dead Assay Kit
英文名称：Cyto3D® Live-Dead Assay Kit
产品货号：BM01
产品规格：1 mL
TheWell BM01 Cyto3D® Live-Dead Assay Kit，产品说明：
Cyto3D® 活死检测试剂盒用于使用快速一步染色程序在双荧光系统上进行分析，从而确定活/死有核细胞。该试剂盒推荐用于 3D、2D 包衣和单层培养细胞的活力分析。
即用型
快
灵敏
非常适合 3D 细胞培养
高性价比
吖啶橙 （AO） 和碘化丙啶 （PI） 都是核染色（核酸结合）染料，用于该试剂盒。AO对活细胞和死细胞都具有渗透性，并对所有有核细胞进行染色以产生绿色荧光。PI仅穿透膜受损的有核细胞的膜，并对死细胞进行染色以产生红色荧光。由于淬灭，当细胞同时用AO和PI染色时，所有活的有核细胞发出绿色荧光，所有死的有核细胞发出红色荧光（PI通过荧光共振能量转移（FRET）降低AO的荧光强度）。红细胞、血小板和碎片等非有核材料不会发出荧光，被荧光显微镜忽略。
使用AO和PI的双荧光活力是推荐的细胞系、原代细胞和干细胞活力分析方法。
The Cyto3D® Live-Dead Assay Kit is used to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating, and on monolayer.
Ready-to-use
Fast
Sensitive
Excellent for 3D cell cultures
Cost-effective
Acridine orange (AO) and propidium iodide (PI), both nuclear staining (nucleic acid binding) dyes, are used in this kit. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. PI only penetrates the membranes of nucleated cells with compromised membranes and stains the dead cells to generate red fluorescence. Due to the quenching, when cells are stained with both AO and PI, all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red (the PI reduces the fluorescence intensity of the AO by fluorescence resonance energy transfer (FRET)). Non-nucleated materials such as red blood cells, platelets and debris do not fluorescence and are ignored by fluorescence microscopes.
Dual-Fluorescence Viability, using AO and PI, is the recommended viability analysis method for cell lines, primary cells, and stem cells.
以下是Thewell品牌部分产品订购信息，如需其他请联系本店客服
| 产品名称 | 货号 | 规格 |
| 25G Precision Tapered Nozzle Tip (50) Non-Sterile | ITN-25G50 | 50 pieces |
| 27G Precision Tapered Nozzle Tip (50) Non-Sterile | ITN-27G50 | 50 pieces |
| 30G Precision Tapered Nozzle Tip (50) Non-Sterile | ITN-30G50 | 50 pieces |
| 22G Needle Tip, 6.35 mm, Non-Sterile (50) | IPN-22G50 | 50 pieces |
| 25G Needle Tip, 6.35 mm Non-Sterile (50) | IPN-25G50 | 50 pieces |
| 27G Needle Tip, 6.35 mm Non-Sterile (50) | IPN-27G50 | 50 pieces |
| 30G Needle Tip, 6.35 mm Non-Sterile (50) | IPN-30G50 | 50 pieces |
| Luer Lock Connector - Female to Female, Sterile (10) | ILL-FF10 | 10 pieces |
| Luer Lock Connector - Female to Female, Sterlie (50) | ILL-FF50 | 50 pieces |
| VitroGel 3D (10 mL) | TWG001 | 10 mL |
| VitroGel 3D (2 mL) | TWG001S | 2 mL |
| VitroGel 3D-RGD (10 mL) | TWG002 | 10 mL |
| VitroGel 3D-RGD (2mL) | TWG002S | 2mL |
| VitroGel RGD (10 mL) | TWG003 | 10 mL |
| VitroGel RGD (2 mL) | TWG003S | 2 mL |
| VitroGel Dilution Solution, TYPE 1 (100 mL) | MS01-100 | 100 mL |
| VitroGel Dilution Solution, TYPE 2 (100 mL) | MS02-100 | 100 mL |
| VitroGel Cell Recovery Solution (100 mL) | MS03-100 | 100 mL |
| VitroGel LDP1 (10 mL) | TWG004 | 10 mL |
| VitroGel COL(10mL) | TWG009 | 10 mL |
| VitroGel LDP1 (2 mL) | TWG004S | 2 mL |
| VitroGel COL(2mL) | TWG009S | 2 mL |
| VitroGel LDP2(10 mL) | TWG005 | 10 mL |
| VitroGel LDP2 (2 mL) | TWG005S | 2 mL |
| VitroGel LDP3 (10 mL) | TWG006 | 10 mL |
| VitroGel LDP3 (2 mL) | TWG006S | 2 mL |
| VitroGel IKVAV (10 mL) | TWG007 | 10 mL |
| VitroGel IKVAV (2 mL) | TWG007S | 2 mL |
| VitroGel YIGSR (10 mL) | TWG008 | 10 mL |
| VitroGel YIGSR (2 mL) | TWG008S | 2 mL |
| VitroGel MMP (10 mL) | TWG010 | 10 mL |
| VitroGel MMP (2 mL) | TWG010S | 2 mL |
| VitroINK 3D (10 mL) | INK01-10 | 10 mL |
| VitroINK 3D (3 mL) | INK01-3 | 3 mL |
| VitroINK RGD (10 mL) | INK02-10 | 10 mL |
| VitroINK RGD (3 mL) | INK02-3 | 3 mL |
| VitroINK 3D-Bioink Starter Kit (10 mL) | ISK01-10 | 1 pack |
| VitroINK 3D-Bioink Starter Kit (3 mL) | ISK01-3 | 1 pack |
| VitroINK RGD-Bioink Starter Kit (10 mL) | ISK02-10 | 1 pack |
| VitroINK RGD-Bioink Starter Kit (3 mL) | ISK02-3 | 1 pack |
| VitroINK Mixing Kit - Starter Pack | IMK00-1 | 1 pack |
| VitroINK Mixing Kit - 1 mL Syringe (1 pack) | IMK01-1 | 1 pack |
| VitroINK Mixing Kit - 1 mL Syringe (10 pack) | IMK01-10 | 10 pack |
| VitroINK Mixing Kit - 3 mL Syringe (1 pack) | IMK03-1 | 1 pack |
| VitroINK Mixing Kit - 3 mL Syringe (10 pack) | IMK03-10 | 10 pack |
| 22G Precision Tapered Nozzle Tip (50) Non-Sterile | ITN-22G50 | 50 pieces |
北京云肽生物科技有限公司
实名认证
金牌会员
入驻年限：3年
