Exo-spin blood 血液类样本外泌体提取试剂盒 EX02

货号： EX02-8，EX02-25，EX02-50
名称：Exo-spin™血液类样本提取试剂盒
成分：沉淀剂，mini SEC纯化管柱
贮存温度：2~8℃
 

产品介绍：

EX02 Exo-spin™ 试剂盒主要用于处理少量血液样本（血清和血浆），包含沉淀剂（buffer）及mini SEC纯化管柱（column）。

Mini Exo-spin™色谱柱和树脂孔径

纯化管柱床体积为500ul，在柱顶部可以加100ul的液体。填充树脂的孔径大约为30nm，以实现高纯度的外泌体洗脱。小于30 nm的所有其他分子（例如蛋白质，脂质）将进入孔中并保留在管柱中。
 

产品数据：

• Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018)

• Data determined by NTA and EXOCET. Serum sample has been analysed for qualitative comparison between Exo-spin™ and precipitation methods.
   

订购信息：

货号	描述
EX02-8	Exo-spin™ blood, 8 columns, 2 ml buffer
EX02-25	Exo-spin™ blood, 24 columns, 25 ml buffer
EX02-50	Exo-spin™ blood, 48 columns, 30 ml buffer

 

下游应用：建议和内容

RNA analysis:

• Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.

• Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery. PLoS One 29;11(4):e0154274.

Mass Spectrometry:

• Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378.

Functional study:

• Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports 24;9(1):608.