8号染色体开放阅读框58抗体

8号染色体开放阅读框58抗体

价格: ¥1080 - 3200

品牌:一研

货号:EY-01K5358

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供应商 :一研

库存 :59

靶点 :说明书

级别 :说明书

目录编号 :说明书

克隆性 :多克隆

抗原来源 :Rabbit

保质期 :说明书

抗体英文名 :C8orf58

抗体名 :8号染色体开放阅读框58抗体

标记物 :Unconjugated

宿主 :说明书

适应物种 :说明书

免疫原 :KLH conjugated synthetic peptide derived from human C8orf58

亚型 :LgG

形态 :液体

应用范围 :说明书

保存条件 :Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

浓度 :1mg/ml

规格 :100ul

抗体制备过程:
1.材料与试剂
  a.提取的动物 Ig
  b.弗氏完全佐剂和弗氏不完全佐剂
  c.青霉素和链霉素
  d.实验动物 兔
  e.其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试,查看免疫效果。
5、如果免疫成功,杀死实验活体,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清(无菌采制)

产品属性:
产品名称 8号染色体开放阅读框58抗体
浓度 1mg/ml
型号 EY-01K5358
产品名称:8号染色体开放阅读框58抗体
英文名称:C8orf58
英文别名:C8orf58; CH058_HUMAN; Chromosome 8 open reading frame 58; Uncharacterized protein C8orf58.

交叉反应: Human, Mouse, Rat
标记: Unconjugated
抗体来源: Rabbit
抗体类型: Polyclonal
免疫原: KLH conjugated synthetic peptide derived from human C8orf58
纯化方法: affinity purified by Protein A
亚型: IgG
性状: Liquid
浓度: 1mg/ml
储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
背景资料: C8orf58 (chromosome 8 open reading frame 58) is a 365 amino acid protein that exists as two alternatively spliced isoforms, which are encoded by a gene that maps to human chromosome 8p21. Consisting of nearly 146 million bases, chromosome 8 encodes about 800 genes. Translocation of portions of chromosome 8 with amplifications of the c-Myc gene are found in some leukemias and lymphomas, and are typically associated with a poor prognosis. Portions of chromosome 8 have been linked to schizophrenia and bipolar disorder. Trisomy 8, also known as Warkany syndrome 2, most often results in early miscarriage but is occasionally seen in a mosaic form in surviving patients who suffer to a varying degree from a number of symptoms including retarded mental and motor development, and certain facial and developmental defects. WRN is a DNA helicase encoded by chromosome 8 and shown defective in those with the early aging disorder Werner syndrome. Chromosome 8 is also associated with Pfeiffer syndrome, congenital hypothyroidism and Waardenburg syndrome.
 


 
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抗体分子标记技术:
(一)原理
当应用化学氧化法时(NaI)遇强氧化剂,离子被氧化为分子,所生成的自由分子可与某些基团进行卤化反应。蛋白质分子可进行卤化反应的基团主要为酪氨酸残基,某些组氨酸残基也可能进行化。在应用胺T( Chloramine T)法的实验中,所用的氧化剂(1,3,4,6- tetrachloro-3a,6adipheny l-glucoluril)强挥发性的有机溶剂中。该溶剂加入试管后,先让其挥发(即让氧化剂将试管包被),然后把Na125I和蛋白质液加入包被好的试管中,反应完成即将混合液移入他管终止反应。
(二)准备
1.试剂
经亲和层析纯化的多克隆抗体或单克隆抗体;0.5mol/L磷酸钠缓冲液,pH7.5;无载体的Na125I3.7GBq/ml(100mCi/ml)的NaOH液;100g/L三醋酸;70%乙醇;新鲜制备的含2mg/m1络胺T的0.5mol/L磷酸钠缓冲液(pH7.5);胺T反应终止缓冲液;
2.仪器
凝胶过滤柱;¥-记数器;玻璃纤维滤。
(三)操作要点
1.用胺T法进行化,也可在大约pH7.0的缓冲液中进行;
2.如果氧化反应损伤蛋白质,可将抗体与异硫酸荧光素(FITC)偶联)胺T量减少到0.02mg/m,并将偏重亚硫酸液的浓度降到0.024mg/ml;
3. Iodogen-包被的试管可在干燥器中,于室温下保存多年;
4.1251标记的抗体,在制备后六周内均可使用(1251的半衰期为59.6天);
5.要注意保证所用的Na1251是新鲜的,陈旧制剂的比活性低;
6.酪氨酸的碘化偶尔会对抗原的抗体结合位点有干扰,因此降低其结合能力,但这种情况很少见。


 

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