ScienCell GK041人体皮肤伤口愈合qPCR检测试剂盒,Human Skin Wound Healing qPCR Array Kit

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英文名 ： ScienCell Human Skin Wound Healing qPCR Array Kit

供应商 ：北京诺博莱德科技有限公司

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约经销商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK041 ScienCell人体皮肤伤口愈合qPCR检测试剂盒，Human Skin Wound Healing qPCR Array Kit，产品简介：
产品品牌:  ScienCell
产品货号：GK041
产品名称：GK041 ScienCell人体皮肤伤口愈合qPCR检测试剂盒，Human Skin Wound Healing qPCR Array Kit
产品规格：One 96-well plate, one 20ul reaction per well


GK041 ScienCell人体皮肤伤口愈合qPCR检测试剂盒，Human Skin Wound Healing qPCR Array Kit， 产品说明：
ScienCell 的 GeneQuery™ 人类皮肤伤口愈合 qPCR 阵列试剂盒 （GQH-SWH） 旨在促进伤口修复过程中涉及的 88 个关键基因的基因表达谱分析。伤口愈合是一个复杂的过程，包括许多生物事件，如吞噬作用、血管生成、新胶原生成、上皮化和胶原蛋白重塑。一般可分为3个阶段：1）止血凝血，2）炎症，3）增殖和组织重塑。如何根据其功能对所包含的基因进行分组的简要示例如下所示：  
伤口愈合阶段
止血和凝血： IL1A， IL1B， IL6， TNF， FGF2， IGF1， TGFs， HIF1A
炎症：CXCL8，VEGFs，ELANE，CTSG，PRTN3，PLAU，TGF，PDGF，TNF，IFNG
增殖和组织重塑：EGF，FGF7，IGF1，IFNG，CSK，RAC1，RHOA，CDC42
细胞外基质成分：COL1A2、COL3A1、COL4A1、COL14A1
细胞粘附：CDH1，ITGA，ITGB，MMP，F3，FGA，TIMP1，ACTA2，ACTC1，TAGLN
细胞因子和趋化因子：CCL2、CXCL1、CXCL2、CXCL8、IL1A、IL1B、IL4、IL6
生长因子：脑脊液2，脑脊液3，EGF，CTGF，FGF，PDGF，TGF
GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有说明，否则引物组可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:     

• Wound healing stages
  • Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A
  • Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG
  • Proliferation and tissue remodeling:  EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42
• Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1
• Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN
• Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6
• Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人体皮肤伤口愈合qPCR阵列板布局*（8 个粗体和斜体控件）

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

COL1A2

CSK

CXCL8

FGF2

IL10

ITGB1

MMP7

PDGFRB

RAC1

TGFBR3

β-actin

B

ACTC1

COL3A1

CTGF

EGF

FGF7

IL1A

ITGB3

MMP9

PLAT

RHOA

TIMP1

GAPDH

C

ANGPT1

COL4A1

CTNNB1

EGFR

FIGF

IL1B

ITGB5

NGF

PLAU

SERPINE1

TNF

LDHA

D

CCL2

COL4A3

CTSG

ELANE

HBEGF

IL4

ITGB6

PDGFA

PLAUR

STAT3

VEGFA

NONO

E

CD40LG

COL5A1

CTSK

F13A1

HGF

IL6

MAPK1

PDGFB

PLG

TAGLN

VEGFB

PPIH

F

CDC42

COL5A3

CTSV

F3

HIF1A

IL6ST

MAPK3

PDGFC

PRTN3

TGFA

VEGFC

GDC

G

CDH1

CSF2

CXCL1

FGA

IFNG

ITGA1

MMP1

PDGFD

PTEN

TGFB1

WISP1

PPC

H

COL14A1

CSF3

CXCL2

FGF10

IGF1

ITGA5

MMP2

PDGFRA

PTGS2

TGFB2

WNT5A

NTC

提示：基因选择可能会根据新的研究和开发进行更新

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