CAS号 :-
供应商 :爱必信(上海)生物科技有限公司
库存 :现货
英文名 :-
保质期 :2-8°C保存。每个组分至少可以保存18个月
保存条件 :2-8°C保存。每个组分至少可以保存18个月
规格 :5mL
包装:5ml(可用于50张片子)
适用:一抗来源于小鼠或兔子
显色底物:DAB
试剂组成:
组分编号 |
组分名称 |
规格 |
数量 |
A |
过氧化氢 |
5ml |
1 |
B |
超级封闭液 |
5ml |
1 |
C |
一抗放大剂 |
5ml |
1 |
D |
酶标二抗聚合物 |
5ml |
1 |
E |
DAB A液 |
5ml |
1 |
F |
DAB B液 |
160ul |
1 |
未提供但必需试剂:
一抗(来源于小鼠或兔子)、PBS (磷酸盐缓冲液)
本试剂盒是基于聚合物技术的一代免疫组化二抗非生物素检测系统,信号更强、操作更加简单。 与传统的免疫组化二抗试剂盒相比,具有3个特点:一是由于聚合物的独到之处,使得酶分子与二抗IgG分子直接结合形成的多聚物分子高度敏感性,有效减少了一抗使用量,一抗试剂可再稀释2-4倍;二是由于避免了生物素的使用,从而可以避免由于生物素引起的非特异性背景显色,得到更清晰的显色和更干净的背景;三是快速,更短的试剂孵育时间。
储存及有效期:
2-8°C保存。每个组分至少可以保存18个月。
操作步骤:
说明:所有的步骤在室温下操作,加试剂可以直接滴瓶滴定,也可以用移液器,一滴=30-40ul
1. 脱蜡、水化组织切片
2. PBS洗2-3次各5分钟
3. 根据所应用的一抗的特殊要求,对组织切片进行预处理
4. PBS洗2-3次各5分钟
5. 加入100ul A 液孵育十分钟,阻断内源性过氧化物酶,以减少非特异性背景染色
6. PBS洗2-3次各5分钟
7. 加入100ul B 液孵育五分钟来减少非特异性染色。注意:此步骤不要超过十分钟;如果一抗在含有5%至10%正常山羊血清的缓冲液中稀释,则此步骤可以省略
8. PBS洗2-3次各5分钟
9. 加一抗,室温或37℃孵育20分钟
10. PBS洗2-3次各5分钟
11. 加入100ul C 液孵育十分钟
12. PBS洗2-3次各5分钟
13. 加入100ul D 液孵育十分钟.注意:此液对光敏感,注意避光
14. PBS洗2-3次各5分钟
15. 配制新鲜底物液:将30ul的 F 液和1ml的 E 液充分混合,即配制成新鲜底物液(该底物液可存放2周)
16. 加入100ul新鲜底物液, 孵育五分钟
17. 去离子水冲洗
18. 复染,封片
该产品被引用文献
- Microglia and astrocytes mediate synapse engulfment in a MER tyrosine kinase-dependent manner after traumatic brain injuryShen Hui, Shi Xiao-Jing, Qi Lin, Wang Cheng, Mamtilahun Muyassar, Zhang Zhi-Jun, Chung Won-Suk, Yang Guo-Yuan, Tang Yao-Hui
Neural Regeneration Research. 2022 Dec 21 .
影响因子: 6.058
- Laminin α1 as a target for the treatment of epidural fibrosis by regulating fibrotic mechanismsPengran Liu, Dan Zhang, Guixiong Huang, Mingdi Xue, Ying Fang, Lin Lu, Jiayao Zhang, Mao Xie, Zhewei Ye
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. 2022 Nov 16 .
影响因子: 5.314
- Intrauterine neuromuscular and stromal dysplasia of the bladder in retinoic acid-induced myelomeningocele fetal ratsLu Li, Liangfeng Tang, Yunli Bi
Tissue and Cell. 2022 Oct 01 .
影响因子: 2.63
- Biomechanical stretch-induced CLOCK upregulation in venous smooth muscle cells promotes phenotypic and functional transformationZhenyu Guo, Wan Zhang, Xu Li, Tao Wang, Xiaohu Yang, Longhua Fan
Vascular Pharmacology. 2022 Oct 01 .
影响因子: 5.738
- PHGDH Inhibitor CBR-5884 Inhibits Epithelial Ovarian Cancer Progression via ROS/Wnt/β-Catenin Pathway and Plays a Synergistic Role with PARP Inhibitor OlaparibXiaocui Zhang, Meige Sun, Yisheng Jiao, Bei Lin, Qing Yang
Oxidative Medicine and Cellular Longevity. 2022 Sep 05 .
影响因子: 7.31
- Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like NeurodegenerationXin Yuan MD,Yingxu Yang MSc,Chaoyang Liu PhD,Ye Tian MD,Danhao Xia MD,Zehua Liu MSc,Lina Pan PhD,Min Xiong PhD,Jing Xiong PhD,Lanxia Meng PhD,Zhaohui Zhang PhD,Keqiang Ye PhD,Haiqiang Jiang PhD,Zhentao Zhang MD, PhD
Movement Disorders. 2022 Aug 22 .
影响因子: 9.698
- Mesenchymal stromal cells pretreated with proinflammatory cytokines enhance skin wound healing via IL-6-dependent M2 polarizationChenyang Liu, Yan Xu, Yichi Lu, Pan Du, Xiaoxiao Li, Chengchun Wang, Peng Guo, Ling Diao, Guozhong Lu
Stem Cell Research & Therapy. 2022 Aug 13 .
影响因子: 8.079
- Impact of dietary sucralose and sucrose-sweetened water intake on lipid and glucose metabolism in male miceXinyi Wu, Le Cui, Haoquan Wang, Jinhong Xu, Zhaozhao Zhong, Xibei Jia, Jiaqi Wang, Huahua Zhang, Yanteng Shi, Yuhang Tang, Qianhui Yang, Qiongdan Liang, Yujing Zhang, Jing Li, Xiaohong Jiang
European Journal of Nutrition. 2022 Aug 07 .
影响因子: 4.865
- A monomeric polysaccharide from Polygonatum sibiricum improves cognitive functions in a model of Alzheimer's disease by reshaping the gut microbiotaShilin Luo, Xin Zhang, Si Huang, Xueping Feng, Xiaojie Zhang, Daxiong Xiang
International Journal of Biological Macromolecules. 2022 Jul 31 .
影响因子: 8.025
- 7,8-Dihydroxyflavone ameliorates mitochondrial impairment and motor dysfunction in the α-synuclein 1–103 transgenic miceYe Tian, Lina Pan, Xin Yuan, Min Xiong, Zhaohui Zhang, Lanxia Meng, Yongfa Zheng, Lihong Bu, Ximing Xu, Zhentao Zhang
Neurobiology of Disease. 2022 Jul 01 .
影响因子: 5.332
- ORF8 protein of SARS-CoV-2 reduces male fertility in miceTing Yu,Qiao Ling,Mengxin Xu,Niu Wang,Lixia Wang,Hanwen Lin,Manqi Cao,Yong Ma,Yuanyuan Wang,Kuibiao Li,Liubing Du,Yunyun Jin,Ying Li,Deyin Guo,Xiaoxue Peng,Yao-qing Chen,Bo Zhao,Ji-An Pan
Journal of Medical Virology. 2022 May 15 .
影响因子: 2.373
- Tau accelerates α-synuclein aggregation and spreading in Parkinson’s diseaseLina Pan, Chunrui Li, Lanxia Meng, Ye Tian, Mingyang He, Xin Yuan, Guoxin Zhang, Zhaohui Zhang, Jing Xiong, Guiqin Chen, Zhentao Zhang
Brain. 2022 May 13 .
影响因子: 11.337
- Persistent activation of Nrf2 in a p62-dependent non-canonical manner aggravates lead-induced kidney injury by promoting apoptosis and inhibiting autophagyCai-Yu Lian, Bing-Xin Chu, Wei-Hao Xia, Zhen-Yong Wang, Rui-Feng Fan, Lin Wang
Journal of Advanced Research. 2022 May 02 .
影响因子: 10.10
- TRAIL-modified, doxorubicin-embedded periodic mesoporous organosilica nanoparticles for targeted drug delivery and efficient antitumor immunotherapyXinyao Feng, Fangshen Li, Liangliang Zhang, Wenmo Liu, Xupu Wang, Rui Zhu, Zhen-An Qiao, Bin Yu, Xianghui Yu
Acta Biomaterialia. 2022 Apr 15 .
影响因子: 10.633
- Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosisGuojing Li, Yu Lin, Yili Zhang, Nihao Gu, Bingxin Yang, Shan Shan, Na Liu, Jing Ouyang, Yisai Yang, Feng Sun, Hong Xu
cell death discovery. 2022 Jan 17 .
影响因子: 4.53
- 宫颈癌LHPP表达情况及其与预后的关系朱明君; 陆晓媛; 方月兰
重庆医学. 2021/07/29 00:00:00 undefined undefined .
- Stroke subtype-dependent synapse elimination by reactive gliosis in miceXiaojing Shi, Longlong Luo, Jixian Wang, Hui Shen, Yongfang Li, Muyassar Mamtilahun, Chang Liu, Rubing Shi, Joon-Hyuk Lee, Hengli Tian, Zhijun Zhang, Yongting Wang, Won-Suk Chung, Yaohui Tang ,Guo-Yuan Yang
Nature Communications. 2021 Nov 26 .
影响因子: 14.919
- ANGPTL4-mediated promotion of glycolysis facilitates the colonization of Fusobacterium nucleatum in colorectal cancerXin Zheng, Rui Liu, Chenchen Zhou, Haopeng Yu, Wanyi Luo, Jianhui Zhu, Jiaxin Liu, Zhe Zhang, Na Xie, Xian Peng, Xin Xu, Lei Cheng, Quan Yuan, Canhua Huang,Xuedong Zhou
Cancer Research. 2021 Aug 11 .
影响因子: 12.701
- Targeting hexokinase 2 increases the sensitivity of oxaliplatin by Twist1 in colorectal cancerBo Zhang,Sze-Hoi Chan,Xue-Qi Liu,Yuan-Yuan Shi,Zhao-Xia Dong,Xin-Rong Shao,Li-Yuan Zheng,Zhi-Ying Mai,Tian-Liang Fang,Li-Zhi Deng,Di-Sheng Zhou,Shu-Na Chen,Miao Li,Xing-Ding Zhang
Journal of Cellular and Molecular Medicine. 2021 Aug 10 ; 18
影响因子: 4.486
- A γ-adducin cleavage fragment induces neurite deficits and synaptic dysfunction in Alzheimer’s diseaseMin Xiong , Li Zou, Lanxia Meng , Xingyu Zhang , Ye Tian , Guoxin Zhang, Jiaolong Yang, Guiqin Chen, Jing Xiong , Keqiang Ye , Zhentao Zhang
Progress in Neurobiology. 2021 Aug 01 .
影响因子: 11.685
- A γ-adducin cleavage fragment induces neurite deficits and synaptic dysfunction in Alzheimer’s diseaseMin Xiong, Li Zou, Lanxia Meng, Xingyu Zhang, Ye Tian, Guoxin Zhang, Jiaolong Yang, Guiqin Chen, Jing Xiong, Keqiang Ye, Zhentao Zhang
Prog Neurobiol. 2021 Aug 01 .
影响因子: 11.68
- 化瘀补肾方对去卵巢小鼠骨折愈合及氧化应激的影响程韶;陶渝仁;舒冰;王晶;赵永见
上海中医药大学学报. 2021 Jul 20 ; 4
- B7-H6 as a Diagnostic Biomarker for Cervical Squamous Cell CarcinomaXuejing Liu, Ruimeng Guo, Yanying Xu
Genetic Testing and Molecular Biomarkers. 2021 Jul 19 .
影响因子: 1.795
- Heterogeneous microenvironmental stiffness regulates pro-metastatic functions of breast cancer cellsChun Liu, Miao Li, Zhao-Xia Dong, Dong Jiang, Xiaojing Li , Shuibin Lin, Demeng Chen, Xuenong Zoua, Xing-Ding Zhang, Gary D. Luker
Acta Biomaterialia. 2021 Jul 08 .
影响因子: 8.947
- pVHL promotes lysosomal degradation of YAP in lung adenocarcinomaLan Hu, Hao Wu, Tian Jiang, Mengzhen Kuang, Bo Liu, Xinying Guo, Daochuan He, Mengqian Chen, Jie Gu, Jianxin Gu, Lei Chang, Mingxiang Feng, Yuanyuan Ruan
Cellular Signalling. 2021 Jul 01 .
影响因子: 4.315
- 咖啡因在帕金森病小鼠模型中神经保护作用的转录组学分析郑余银, 冯艺佳, 阮青青, 屠文展
温州医科大学学报. 2021 Jun 01 .
- Pinin promotes tumor progression via activating CREB through PI3K/AKT and ERK/MAPK pathway in prostate cancerXiang-Yu Meng, Hui-Zhi Zhang, Yi-Yue Ren, Ke-Jie Wang, Jun-Feng Chen, Rui Su, Jun-Hui Jiang, Ping Wang, Qi Ma
Am J Cancer Res. 2021 Apr 15 ; 4
影响因子: 4.918
- Novel biomarkers of intervertebral disc cells and evidence of stem cells in the intervertebral discJ.Wang,Y.Huang,L.Huang,Shi,J.Wang,Zhu,Li‡L.Zhang,G.Feng,L.Liu,Y.Song
Osteoarthritis and Cartilage. 2021 Mar 03 .
影响因子: 6.576
- 3M综合征相关蛋白和LC3蛋白在正常小鼠生长板表达的时空差异性和意义付鹏九
河北医科大学. 2021 Mar 01 .
- Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXLXunlei Pang, Yanhong Wang, Bei Miao, Sujuan Fei, Weichang Chen
Oncology Letters. 2021 Mar 01 ; 3
影响因子: 2.967
- Therapeutic effect of the injectable thermosensitive hydrogel loaded with SHP099 on intervertebral disc degenerationJingcheng Wang, Leizhen Huang, Yong Huang, Yulin Jiang, Li Zhang, Ganjun Feng, Limin Liu
Life Sciences. 2021 Feb 01 .
影响因子: 2.685
- Inflammatory cell-derived CXCL3 promotes pancreatic cancer metastasis through a novel myofibroblast hijacked cancer escape mechanismXiaoting Sun,Xingkang He, Yin Zhang, Kayoko Hosaka,Patrik Andersson,Jing Wu, Jieyu Wu,Xu Jing, Qiqiao Du,Xiaoli Hui, Bo Ding,Ziheng Guo,An Hong,Xuan Liu,Yan Wang,Qing Ji,Rudi Beyaert, Yunlong Yang,Qi Li, Yihai Cao
Gut. 2021 Jan 20 .
影响因子: 23.059
- Human Umbilical Cord Blood Derived-Mesenchymal Stem Cells Alleviate Dextran Sulfate Sodium-Induced Colitis by Increasing Regulatory T Cells in MiceYing Li, Ke Ma, Luping Zhang, Hong Xu, Nan Zhang
Front. Cell Dev. Biol.. 2020 Nov 24 .
影响因子: 5.186
- High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinomaXiao-Yang Hua, Xing-Xing Bie, Xi Cheng, Shu-Guang Zhang
Molecular Medicine Reports. 2020 Nov 03 ; 1
影响因子: 2.100
- Human osteoarthritis cartilage-derived stromal cells activate joint degeneration through TGF-beta lateral signalingWenguang Liu, Meng Feng, Chathuraka T. Jayasuriya, Hang Peng, Long Zhang, Yingjie Guan, John A. Froehlich, Richard M. Terek, Qian Chen
FASEB JOURNAL. 2020 Oct 29 ; 12
影响因子: 4.718
- Asparagine endopeptidase inhibitor protects against fenpropathrin-induced neurodegeneration via suppressing α-synuclein aggregation and neuroinflammationTing Yu, Fang Wan, Chaoyang Liu, Xingyu Zhang, Zehua Liu, Jichun Zhang, Jing Xiong, Tao Wang, Zhentao Zhang
European Journal of Pharmacology. 2020 Oct 05 .
影响因子: 3.263
- Airway Epithelial Hepcidin Coordinates Lung Macrophages and Immunity Against Bacterial PneumoniaYang Yang, Zeng CongLi, Yang ShiYue, Zhang Yan, Song ShengWen, Liu SiJin, Shu Qiang, Fang XiangMing, Chen QiXing
Shock. 2020 Sep 01 ; 3
影响因子: 2.55
- Synergistic effect of Huyang Yangkun Formula and embryonic stem cells on 4-vinylcyclohexene diepoxide induced premature ovarian insufficiency in miceMeifang Li, Li Xie, Yang Li, Jian Liu, Guangning Nie,Hongyan Yang
Chinese Medicine. 2020 Aug 05 .
影响因子: 5.455
- Abscisic Acid-Induced Autophagy Selectively via MAPK/JNK Signalling Pathway in GlioblastomaNan Zhou, Zixuan Wei, Zengxin Qi, Liang Chen
Cellular and Molecular Neurobiology. 2020 Jun 23 .
影响因子: 3.606
- 薄荷醇缓解胃食管反流的机制研究及技术研发张志明
江南大学. 2020 Jun 01 .
- SLC26A3转运体(DRA)在溃疡性结肠炎中增强肠上皮细胞屏障的作用研究丁向明
华中科技大学. 2020 Jun 01 .
- 靶向肿瘤血管的溶瘤腺病毒构建及感染性质分析李哲
吉林大学. 2020 Jun 01 ; 8
- MIF inhibitor, ISO-1, attenuates human pancreatic cancer cell proliferation, migration and invasion in vitro, and suppresses xenograft tumour growth in vivoBo Cheng, Qiaofang Wang, Yaodong Song, Yanna Liu, Yanyan Liu, Shujun Yang, Dejian Li, Yan Zhang,Changju Zhu
Scientific Reports. 2020 Apr 21 .
影响因子: 4.379
- VX-765 attenuates atherosclerosis in ApoE deficient mice by modulating VSMCs pyroptosisYiyi Li, Xuan Niu, Haitao Xu, Qingwen Li, Lanxia Meng, Mingyang He, Jie Zhang, Zhentao Zhang, Zhaohui Zhang
Experimental Cell Research. 2020 Apr 01 ; 1
影响因子: 3.905
- Screening and Identification of Potential Biomarkers for Hepatocellular Carcinoma: An Analysis of TCGA Database and Clinical ValidationXianli Wei, Junzi Ke, Haonan Huang, Shikun Zhou, Ao Guo, Kun Wang, Yujuan Zhan, Cong Mai, Weizhen Ao, Fuda Xie, Rongping Luo, Jianyong Xiao, Hang Wei, Bonan Chen
Cancer Manag Res. 2020 Mar 17 .
影响因子: 2.243
- Neonatal exposure to bisphenol A advances pubertal development in female ratsJing Qiu,Yanyan Sun,Wen Sun,Yonghong Wang,Teng Fan,Jian Yu
Molecular reproduction Development. 2020 Feb 28 ; 4
影响因子: 2.124
- Menthol relieves acid reflux inflammation by regulating TRPV1 in esophageal epithelial cellsZhiming Zhang, Xiaoli Wu, Liwen Zhang, Aiqin Mao, Xin Ma, Dongxu He
BIOCHEM BIOPH RES CO. 2020 Feb 17 .
影响因子: 2.705
- Laminin α5 modulates fibroblast proliferation in epidural fibrosis through the PI3K/AKT/mTOR signaling pathwayPengran Liu, Hui Chen, Lianqi Yan, Yu Sun
Molecular Medicine Reports. 2020 Jan 28 .
- p38α Mitogen-Activated Protein Kinase Is a Druggable Target in Pancreatic AdenocarcinomaLing Yang, Xiaoting Sun, Ying Ye, Yongtian Lu, Ji Zuo, Wen Liu, Adrian Elcock, Shun Zhu
Frontiers in Oncology. 2019 Nov 26 .
影响因子: 4.606
- Expression of GLS1 in intrahepatic cholangiocarcinoma and its clinical significanceJun Cao,Chi Zhang,Guo‑Qing Jiang,Sheng‑Jie Jin,Zhi‑Hui Gao,Qian Wang,De‑Cai Yu,Ai‑Wu Ke,Yi‑Qun Fan,Da‑Wei Li,Ao‑Qing Wang,Dou‑Sheng Bai
Mol Med Rep. 2019 Jun 19 .
影响因子: 1.922
- 乳腺癌患者的D人格与分子分型及预后的相关性分析张宁
华北理工大学. 2019 May 24 .
- A7细胞群去甲肾上腺素能神经元对慢性间歇低氧大鼠颏舌肌中枢调控的影响聂昕时
中国医科大学. 2019 Mar 01 .
- 唾液酸转移酶ST3Gal3在子宫内膜接受态建立中的作用研究王浩
大连医科大学. 2019 Mar 01 ; 4
- 乳腺癌分子分型与人格特征的关系张宁;赵月;苏楠;杨乐;李英杰
癌症进展. 2019 Feb 25 .
- lncRNA PLAC2 activated by H3K27 acetylation promotes cell proliferation and invasion via the activation of Wnt/β‑catenin pathway in oral squamous cell carcinomaFubo Chen, Shengcai Qi, Xu Zhang, Jinjin Wu, Xi Yang, Raorao Wang
International Journal of Oncology. 2019 Feb 01 ; 4
影响因子: 5.65
- Chrysin protects against renal ischemia reperfusion induced tubular cell apoptosis and inflammation in miceMingwei Xu,Hongbo Shi,Dongcao Liu
EXP THER MED. 2019 Jan 21 .
影响因子: 1.410
- TMEM206通过与AKT和细胞外信号调节激酶信号通路相互作用促进结直肠癌细胞的恶性表型赵劲博
中国医科大学. 2018 Aug 01 ; 2