ScienCellR8918大鼠端粒长度定量qPCR分析试剂盒(绝对定量)ARTLQ,Absolute Rat Telomere Length Quantification qPCR Assay Kit
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英文名 :ScienCell Absolute Rat Telomere Length Quantification qPCR Assay Kit
供应商 :北京诺博莱德科技有限公司
R8918 ScienCell 大鼠端粒长度定量qPCR分析试剂盒(绝对定量)ARTLQ,Absolute Rat Telomere Length Quantification qPCR Assay Kit
ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。
北京诺博莱德作为ScienCell品牌的特约经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
R8918 ScienCell大鼠端粒长度定量qPCR分析试剂盒(绝对定量)ARTLQ,Absolute Rat Telomere Length Quantification qPCR Assay Kit,产品简介:
产品品牌; ScienCell
产品货号:R8918
产品名称:R8918 ScienCell大鼠端粒长度定量qPCR分析试剂盒(绝对定量)ARTLQ,Absolute Rat Telomere Length Quantification qPCR Assay Kit
产品规格:100 reactions
我们提供较快和较敏感的细胞分析检测,用于高通量的生物医学研究、药物筛选和诊断领域。基于细胞的检测是必要的、有用的临床实验室研究工具。ScienCell研究实验室已经开发出多种的细胞检测试剂盒,用于不同领域的研究,包括代谢、氧化应激、酶活性分析等。
R8918 ScienCell大鼠端粒长度定量qPCR分析试剂盒(绝对定量)ARTLQ,Absolute Rat Telomere Length Quantification qPCR Assay Kit,产品说明:
端粒是染色体末端的重复核苷酸元件,可保护染色体免受降解和遗传信息丢失。正常的二倍体细胞在每个细胞周期中失去端粒。因此,端粒长度会随着时间的推移而减少,并可能预测寿命。端粒缩短对健康状况有负面影响,并与许多健康问题有关,包括衰老和癌症。端粒长度的准确和一致定量在细胞生物学的许多方面都很重要,例如染色体不稳定、DNA 修复、衰老、细胞凋亡、细胞功能障碍和肿瘤发生。
ScienCell 的大鼠绝对端粒长度定量 qPCR 检测试剂盒 (ARTLQ) 旨在直接测量大鼠细胞群的平均端粒长度。端粒引物集识别并扩增端粒序列。单拷贝参考(SCR)引物集识别并扩增大鼠17号染色体上100 bp长的区域,并作为数据归一化的参考。已知端粒长度的参考基因组DNA样品可作为计算目标样品端粒长度的参考。精心设计的引物可确保:(i)可靠定量的高效率;(ii)无非特异性扩增。每个引物组都经过qPCR验证,包括熔解曲线分析和凝胶电泳,以提高扩增特异性,并通过模板连续稀释法验证扩增效率。
Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.
ScienCell's Absolute Rat Telomere Length Quantification qPCR Assay Kit (ARTLQ) is designed to directly measure the average telomere length of a rat cell population. The telomere primer set recognizes and amplifies telomere sequences. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on rat chromosome 17, and serves as reference for data normalization. The reference genomic DNA sample with known telomere length serves as a reference for calculating the telomere length of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.
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