库存 ：充足

英文名 ：Lipofectamine™ 2000

CAS号 ：无

保质期 ：详见说明

供应商 ：杭州昊鑫生物

保存条件 ：2℃-8℃

规格 ：0.75ml

Lipofectamine™ 2000 转染试剂

Lipofectamine™ 2000转染试剂是一种多用途转染试剂，可在各种贴壁和悬浮细胞系中提供高效转染。研究人员可使用Lipofectamine™ 2000试剂进行基于siRNA和shRNA的基因敲除实验及基因表达研究。Lipofectamine™ 2000是Life Technologies提供的用于siRNA和质粒DNA共转染的最佳试剂。


采用Lipofectamine™ 2000试剂，您可以实现：
• 在各种细胞系中具有卓越的转染效率和高重组蛋白表达水平 (表1)
• 出众的siRNA和质粒DNA共转染性能
• 在血清存在时亦具有可靠的转染效率 – 转染后无需更换培养基
• 适用于高通量应用的可靠性能
• 建立稳定细胞系的最佳选择

适用于基因表达和基因沉默的高性能转染试剂
Lipofectamine™ 2000试剂适合所有常见细胞系及许多难以转染的细胞系，可用于含有或不含血清的培养基。在基因沉默应用中，Lipofectamine™ 2000试剂的高效转染提供了很高的基因敲除水平，足以获得令人信服的结果。Lipofectamine™ 2000试剂可实现siRNA和质粒DNA的高效转染，它是共转染的试剂。Lipofectamine™ 2000试剂使用方便 – 只需将其与核酸混合，加入细胞培养物即可。

Lipofectamine®2000转染方案/质粒转染



脂质体2000转染示例（如下图）：

 

引用和文献：

Molecular and Biochemical Characterization of Rat epsilon -N-Trimethyllysine Hydroxylase, the First Enzyme of Carnitine Biosynthesis.

• 作者:Vaz F M; Ofman R; Westinga K; Back J W; Wanders R J;
• 期刊:J Biol Chem
• PubMed ID:11431483

A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma.

• 作者:Sarkar Shubhashish; Roy Badal Chandra; Hatano Naoya; Aoyagi Teiichiro; Gohji Kazuo; Kiyama Ryoiti;
• 期刊:J Biol Chem
• PubMed ID:12133830

Naturally occurring mutations in the largest extracellular loops of ABCA1 can disrupt its direct interaction with apolipoprotein A-I.

• 作者:Fitzgerald ML, Morris AL, Rhee JS, Andersson LP, Mendez AJ, Freeman MW.
• 期刊:J Biol Chem
• PubMed ID:12084722

The identification and characterization of a noncontinuous calmodulin-binding site in noninactivating voltage-dependent KCNQ potassium channels.

• 作者:Yus-Najera Eva; Santana-Castro Irene; Villarroel Alvaro;
• 期刊:J Biol Chem
• PubMed ID:12032157

cAMP-dependent protein kinase regulates desensitization of the capsaicin receptor (VR1) by direct phosphorylation.

• 作者:Bhave Gautam; Zhu Weiguo; Wang Haibin; Brasier D J; Oxford Gerry S; Gereau Robert W 4th;
• 期刊:Neuron
• PubMed ID:12194871

Human apical sodium-dependent bile salt transporter gene (SLC10A2) is regulated by the peroxisome proliferator-activated receptor alpha.

• 作者:Jung Diana; Fried Michael; Kullak-Ublick Gerd A;
• 期刊:J Biol Chem
• PubMed ID:12055195

The activated glucocorticoid receptor modulates presumptive autoregulation of ribosomal protein S6 protein kinase, p70 S6K.

• 作者:Shah O Jameel; Iniguez-Lluhi Jorge A; Romanelli Angela; Kimball Scot R; Jefferson Leonard S;
• 期刊:J Biol Chem
• PubMed ID:11705993

Transcriptional Regulation of Kaposi's Sarcoma-associated Herpesvirus-encoded Oncogene Viral Interferon Regulatory Factor by a Novel Transcriptional Silencer, Tis.

• 作者:Wang Xin-Ping; Zhang Yan-Jin; Deng Jian-Hong; Pan Hong-Yi; Zhou Fu-Chun; Gao Shou-Jiang;
• 期刊:J Biol Chem
• PubMed ID:11821384

A highly effective dominant negative alpha s construct containing mutations that affect distinct functions inhibits multiple Gs-coupled receptor signaling pathways.

• 作者:Berlot Catherine H;
• 期刊:J Biol Chem
• PubMed ID:11927592