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库存 :大量
英文名 :MG Phosphate Assay Kit *Colorimetric*
CAS号 :MG Phosphate Assay Kit *Colorimetric*
保质期 :详见说明
供应商 :齐源生物
保存条件 :详见说明
规格 :1 kit
MG 磷酸盐检测试剂盒比色法、MG Phosphate Assay Kit *Colorimetric*详细如下:MG(孔雀石绿)磷酸盐检测试剂盒基于对孔雀石绿、钼酸盐和游离zheng磷酸盐之间形成的绿色复合物的定量分析。反应的快速颜色形成可以方便地在分光光度计 (600-660 nm) 或读板器上测量。非放射性比色测定试剂盒经过优化,可提供卓越的灵敏度和延长的保质期。该测定方法简单快速,只需一个添加步骤即可测定磷酸盐。测定可以在试管、比色皿或多孔板中进行。该测定可以方便地在 96 或 384 孔板中进行,用于酶抑制剂的高通量筛选。该试剂盒已用于测定磷酸酶、磷脂酶或脂肪酶、核苷三磷酸酶(ATP酶、GTP酶等)和植酸酶。
Phosphate standard: Add 25 L of 1 mM phosphate standard (Component A) in 475 uL of deionized water or enzyme reaction buffer to get 50 M phosphate solution. Then take 200 L of 50 M phosphate solution to perform 2-fold serial dilutions to get 25,12.5, 6.25, 3.125, 1.56, and 0.78 M phosphate solutions. Include a blank control.
Add 80 L of test sample into microplate wells.
Note 1: Use deionized water to dilute test samples.
Note 2: If the samples are diluted in buffers containing substances that may affect assay performance, test
the same amount of that buffer with phosphate standards.
Set up phosphate standard: Add 80uL serially diluted phosphate standard solution
(from Step 1.1) to the wells.
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