抗-大鼠 MOG (1-125) IgG 定量 ELISA 试剂盒比色法

库存 ：大量

英文名 ：Anti-Rat MOG (1-125) IgG Quantitative ELISA Kit

CAS号 ：Anti-Rat MOG (1-125) IgG Quantitative ELISA Kit

保质期 ：详见说明

供应商 ：齐源生物

保存条件 ：-20°C

规格 ：1 kit

抗-大鼠 MOG (1-125) IgG 定量 ELISA 试剂盒比色法、Anti-Rat MOG (1-125) IgG Quantitative ELISA Kit详细如下：
Anti-Rat MOG (1-125) IgG 定量 ELISA 试剂盒为抗大鼠 MOG (1-125) 自身抗体提供了一种方便的定量检测方法。这种比色法有助于研究人员确定存在的抗大鼠 MOG (1-125) 抗体的量，并有助于提供有关其在多发性硬化症发病机制动物模型 EAE 的发展和治疗中所起作用的信息。检测限：1纳克/毫升、主持人：大肠杆菌
1 Establish dilution range of serum samples: Serial dilutions of serum samples can start from
1:1k, 1:5k, 1:25k, 1:125k. Use 1X Sample Dilution Buffer (Component C) to do the dilution (an
example is shown in Table 1). Depending on the amount of antibody present, the dilution range
can be further adjusted.
2 Arrange and label strips (Component A) based on the number of wells with standard and
samples. An example is shown in Table 1. Although diluted standard and samples can be run
as single points, duplicates are recommended.
3 Dilute anti-rat MOG (1-125) IgG standard (Component B) in 1X Sample Dilution Buffer
 (Component C) according to the Table 2.
4 Add 100 μl of the diluted standards into wells (A1-G2 for duplicate run). Add 100 μl of 1X
Sample Dilution Buffer (Component C) as a blank into wells H1-H2.
5 Add diluted samples into appropriate wells (depends on the number of samples to be tested).
 After adding the standards and samples to the wells, cover the plate and incubate at room
 temperature for 60 min with gentle shaking.
6 Prepare 1X working wash buffer by diluting the 10X Wash Buffer (Component D) with DI H2O.
7 Wash wells five times at 200 μl/well of 1X washing buffer. Pat dry.
8 Dilute goat anti-Rat IgG-HRP (Component G) secondary antibody (2nd Ab) with sample dilution
buffer (component C): working solution at 1:2,000 dilution. Add 100 μl of the diluted 2nd Ab into
each well; incubate plate at room temperature for 45-60 min with gentle shaking.
9 Wash wells five times with 200 μl per well of 1X washing buffer. Pat dry. Clean the outside
 bottom of the wells with lens paper if necessary before the next step (this ensures accurate
 absorbance reading).
10 Add 100 μl of the TMB color substrate solution (Component E) into each well. Tap plate gently
 and incubate at room temperature until blue gradient is clearly observed across the wells (1-15
 min). It may be necessary to adjust color development time so that absorbance values fall
 within the detection range.
11 Add 50 μl of the Stop Solution (Component F) into each well and tap plate gently (blue color will
 turn to yellow). Measure absorbance (OD) at 450 nm using a microplate absorbance reader
 within 20 minutes after adding stop solution.