重组人泛素活化酶E1(UBE1) Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1)

价格： ￥1920

品牌：翌圣生物（Yeasen）品牌认证

货号：20433ES25

产品详情

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库存：大量

英文名：Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1)

CAS号：点击查看

保质期：有效期1年

供应商：翌圣生物科技（上海）股份有限公司

保存条件：-20℃保存

规格：25 µg

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.

产品性质

别名(Synonyms)	UBA1, UBA6
来源(Source)	E.coli
分子量 (Molecular Weight)	Approximately 118 kDa.
氨基酸序列 (AA Sequence)	MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
外观 (Physical Appearance)	Liquid
标签(Tag)	None
纯度(Purity)	> 95% by SDS-PAG
缓冲液(Buffer)	50mM PBS, pH7.0, 150mM NaCl, 1mM DTT.

储存条件
-25~-15℃保存

注意事项
1. 避免反复冻融。
2. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
反应缓冲液配方

名称	组分	浓度
10 × Reaction Buffer（200 ul）	Tris PH8.0	500 mM
	DTT	6 mM
	ddH2O	to 200μL
10 x ATP-Mg （200 ul）	ATP	100 mM
	MgCl2	100 mM
	ddH2O	to 200μL

参考阳性反应体系

组分	体积 (μL)
ddH2O	to 20 μL
10 × Reaction Buffer	2 μL
10 × E1 Enzyme	2 μL
5 × Ubiquitin	4 μL
10 × ATP-Mg	2 μL
20 × E2 Enzyme	1 μL
10 × E3 Ligase Itch (Positive control)	2 μL
20 × S5a substrate (Positive control)	1 μL