Ras-GTP

价格： ￥4800

品牌：Bioyears

货号：TD-26909

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供应商：Bioyears

库存：88

靶点：Ras

级别：科研级

目录编号：TD-26909

克隆性：单克隆

抗原来源：蛋白表达

保质期：24个月

抗体英文名： Anti-Ras-GTP Mouse Monoclonal Antibody

抗体名：Ras-GTP

标记物：FITC

宿主：Mouse

适应物种：脊椎动物

免疫原：Recombinant full length protein of active H-Ras

亚型：IgG

形态：液体

应用范围：IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

保存条件：-20℃

浓度：1mg/ml

规格：30μl

Ras-GTP

Cat. #: TD-26909

Size: 30 µL

Gene Symbol: RAS

Description: Anti-Ras-GTP Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Immunogen: Recombinant full length protein of active H-Ras

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-Ras-GTP monoclonal antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:

IP/WB Analysis of Active Ras Protein.Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Ras-GTP monoclonal antibody (Cat. # TD-26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. # ).