英文名 ：SARS-CoV-2 S-trimer Protein (Mammalian, D614G, C-6His)

Recombinant SARS-CoV-2 S-trimer Protein (D614G, C-6His)

 

Catalog #:  DRA59       Derived From: Human Cells   

 

Description:

Recombinant SARS-CoV-2 S-trimer Protein is produced by our Mammalian expression system and the target gene encoding Cys15-Gln1208(D614G) is expressed with a 6His tag at the C-terminus. Two proline substitutions and furin  cleavage site substitutions are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Accession:  QHD43416.1

Names(Known as): SARS-CoV-2 S protein; SARS-CoV-2 Spike glycoprotein; SARS-CoV-2 S glycoprotein; SARS-nCoV-2 Spike glycoprotein

 

Quality Control:

Mol Mass:  136.5kDa      AP Mol Mass:  170-220kDa, reducing conditions

Purity:  Greater than 95% as determined by reducing SDS-PAGE.

Purity:  Greater than 90% as determined by reducing SEC-HPLC.	------	Purity:  Greater than 90% as determined by reducing SEC-HPLC.

Bioactivity:

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Immobilized SARS-CoV-2 S-trimer (D614G)-His (Cat#DRA59) at 2μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#C05Y). The ED50 of Recombinant Human ACE-2-Fc(Cat#C05Y) is 31.75 ng/ml.		Immobilized SARS-CoV-2 S-trimer (D614G)-His (Cat#DRA59) at 2μg/ml (100 μl/well) can bind Anti-SARS-CoV-2 S1 mAb (5D9)(Cat#NC025). The ED50 of Anti-SARS-CoV-2 S1 mAb (5D9)(Cat#NC025) is 15.81 ng/ml.

 

Formulation:

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

 

Shipping:

The product is shipped on dry ice pack.

Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at ≤ -20°C.

 

Purification: 
Affinity purification chromatography.

 

Application:
Immunogen, calibrator or standard.

 

Background:

The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY