英文名 ：Human NSE (诊断原料,神经生物学）

Recombinant Human NSE 

 

Catalog #:  DRA01        Derived From: E.coli   

 

Description:

Recombinant Human NSE is produced by E.coli expression system and the target gene encoding Met1-Leu434 is expressed with a 6His tag at the N-terminus.

Accession:  P09104

Names(Known as):Enolase; NSE;Neuron Specific Enolase; enolase; 2-phosphoglycerate dehydratase;nervous-system specific enolase; phosphoenolpyruvate hydratase;2-phosphoglyceric dehydratase; 2-phosphoglycerate enolase; γ-enolase

 

Quality Control:

Mol Mass:  49.4kDa           AP Mol Mass:  55kDa, reducing conditions

Purity:  Greater than 95% as determined by reducing SDS-PAGE.

 

Formulation:

Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM KCl, 5mM MgSO₄, pH7.5.

 

Shipping:

The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at ≤ -20°C.

 

Purification: 
Affinity purification chromatography.

 

Immunoreactivity: 
N.A.

 

Application:
Immunogen, calibrator or standard.

 

Background:

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

 

 

References:

In-situ engineered MXene-TiO2/ BiVO4 hybrid as an efficient photoelectrochemical platform for sensitive detection of soluble CD44 proteins[J]. Razium Ali Soomro. et al. Biosensors and Bioelectronics. 2020.

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY