提供商 :普健生物
服务名称 :高通量低内毒素重组抗体表达
服务简介
普健生物拥有自主研发的XtenCHOTM哺乳表达系统 , 能高效快速表达各种类型的抗体和重组蛋白。 高通量低内毒抗体表达平台是大量生产抗体小样经济实惠的选择, 表达体系从1ml至30ml,一次性可以同时进行200个样本的转染生产。 从基因合成到抗体纯化,再到完善的抗体质量检测系统(SDS-PAGE, Elisa, SEC-HPLC, Biacore,DSC, Octet,FACS 等),普健生物经过近十多年的技术积累能提供一站式服务及个性化设计,形成了完善的高通量质粒大提,重组单克隆抗体表达和低内毒素抗体纯化平台,纯化的重组抗体内毒素含量可低至0.1 EU/ml,为抗体筛选及抗体药开发提供便捷高效安全的服务。
服务优势
1.拥有丰富的重组抗体生产经验,自主开发的XtenCHOTM哺乳表达体系,产量高, 已经生产上万种抗体。
2.产能高, 一次性可以同时进行200个抗体的转染,生产周期快。
3.低内毒,纯化后重组抗体内毒素含量可低至0.1 EU/ml
服务内容
服务内容 |
客户提供 |
服务步骤 |
服务周期 |
交付内容 |
高通量低内毒抗体表达 |
抗体序列 |
序列优化与基因合成 |
2-3周 |
目的抗体 技术服务报告 |
无内毒素质粒大提 |
细胞转染与培养 |
低内毒素抗体纯化 |
内毒素含量测定 |
案例展示
普健生物(武汉)科技有限公司于2012年在武汉成立,作为武汉国家生物产业基地指定的光谷抗体发现与筛选公共服务平台,具备优越的技术实力和国际化发展的战略格局,专注于重组蛋白、抗体、诊断原料、抗体药物发现等自主技术平台的建设和下游生物医药产品的战略合作。
公司大楼坐落于东湖新技术开发区神墩四路, 这里绿叶繁茂,道路宽敞,周围有一众生物科技型高新企业。普健生物作为一员加入此处,为周遭增添了一抹亮丽的色彩。
公司立足于生命科学领域,自主建立三大完善的技术服务平台:生命科学研究平台、诊断原料开发平台、抗体药物研发平台。生命科学平台:拥有五大成熟高效的蛋白表达系统(枯草芽孢杆菌、大肠杆菌、酵母、昆虫细胞、哺乳动物细胞),鼠/兔单克隆抗体平台,可提供蛋白表达、分子构建-蛋白表达纯化-结构解析、抗体重组表达、鼠/兔单克隆抗体制备以及相关检测服务。诊断原料开发平台:拥有开放式化学发光自动平台,可缩短研发周期、降低开发成本与技术风险,为广大客户提供各种诊断核心原料开发。抗体药物发现平台以杂交瘤筛选与噬菌体展示技术为主,目前已完成构建并对外提供抗体筛选技术服务的文库有:新冠特异性抗体噬菌体文库、羊驼天然纳米抗体噬菌体文库、兔源天然抗体噬菌体文库、小鼠天然抗体噬菌体文库。同时也开发了大量的重组蛋白、抗体、工具酶、药物对照抗体、工业酶、诊断原料等相关产品。
目前已拥有的自主技术包括:哺乳动物细胞重组蛋白表达系统、稳定细胞株构建、噬菌体文库的构建以及淘选、DNA免疫技术、化学发光免疫诊断抗体筛选技术等等。截止目前已成功交付数万例与重组蛋白、抗体相关的技术委托研发,客户以及合作伙伴涵盖了国内外优秀的学术研究机构以及制药公司;并且与FIND(创新诊断基金会)、BAYER(拜耳作物科学)、广州呼吸健康研究院建立合作关系;与青岛大学附属医院医学研究转化中心,苏州系统医学研究所,广州再生医学与健康广东省实验室建立战略合作关系。
该产品被引用文献
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- Evaluation of differentially expressed microRNAs in vitrified oocytes by next generation sequencing
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- Protein profile of Dabry’s Sturgeon (Acipenser dabryanus) spermatozoa and relationship to sperm quality
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- Cyclin D1 silencing impairs DNA double strand break repair, sensitizes BRCA1 wildtype ovarian cancer cells to olaparib
- SOX2 inhibits cell proliferation and metastasis,promotes apoptotic by downregulating CCND1 and PARP in gastric cancer
- Variability in the protein profiles in spermatozoa of two sturgeon species
- Constitutive activation of STAT3 and cyclin D1 overexpression contribute to proliferation,migration and invasion in gastric cancer cells
- "Individual phosphorylation sites at the C-terminus of the apelin receptor play different roles in signal transduction"
- 11 β ‑hydroxysteroid dehydrogenase‑1 is associated with the activation of hepatic stellate cells in the development of hepatic fibrosis
- Pyk2/MCU Pathway as a New Target for Reversing Atherosclerosis
- An optimized and robust SARS-CoV-2 pseudovirus system for viral entry research
- Multi-omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis
- A Sensitive and High-Throughput Flow Cytometry-Based Assay for Measuring Antibody Neutralization of Human Adenovirus Type 3
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- ENAM gene associated with T classification and inhibits proliferation in renal clear cell carcinoma
- "A Sensitive and High-Throughput Flow Cytometry-Based Assay for Measuring Antibody Neutralization of Human Adenovirus Type 3"
- Identification of ALDH3A2 as a novel prognostic biomarker in gastric adenocarcinoma using integrated bioinformatics analysis
- Cutting Edge: EPHB2 Is a Coreceptor for Fungal Recognition and Phosphorylation of Syk in the Dectin-1 Signaling Pathway
- Shrimp Plasma MANF Works as an Invertebrate Anti-Inflammatory Factor via a Conserved Receptor Tyrosine Phosphatase
- Multiple SARS-CoV-2 Variants Exhibit Variable Target Cell Infectivity and Ability to Evade Antibody Neutralization
- Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells
- Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice
- "Antibody engineering improves neutralization activity against K417 spike mutant SARS-CoV-2 variants"
- Effects of β-Asarone on Ischemic Stroke in Middle Cerebral Artery Occlusion Rats by an Nrf2-Antioxidant Response Elements (ARE) Pathway-Dependent Mechanism