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库存 :大量
英文名 :His-Spin Protein Miniprep
CAS号 :见产品标签
保质期 :根据订购时间与产品批次判定,欢迎联系百奥创新咨询确认
供应商 :北京百奥创新科技有限公司
保存条件 :Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.
规格 :具体价格请联系我们
产品描述:The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.Affinity Matrix:Nickel charged agarose
Binding Capacity:1 mg
Elution Method:Imidazole gradient
Elution Volume:100 - 200 µl (150 µl is optimal)
Principle of Technology:Based on a nickel-charged His-Affinity Gel (IMAC).
Processing Time:5 minutes
Protein Purity:Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
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