pLPCX逆转录病毒载体

库存 ：999

英文名 ：pLPCX

CAS号 ：见包装

保质期 ：见包装

供应商 ：HonorGene

保存条件 ：见包装

规格 ：5ug质粒+1ml甘油菌

产品参数：

质粒类型：逆转录病毒载体

启动子：CMV

载体大小：6265bp

5' 测序引物及序列：LNCX:5'd[AGCTCGTTTAGTGAACCGTCAGATC]3'

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Puromycin (嘌呤霉素)

载体介绍：

pLPCX contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus(MoMuSV), and is designed for retroviral gene delivery and expression. Upon transfection into a packaging cell line, pLPCX transiently expresses, or integrates and stably expresses, a transcript containing Ψ+ signal) the puromycin resistance gene, and the gene of interest. The 5' viral LTR in this vector contains promoter/enhancer sequences that control expression of the puromycin resistance(Puro+) gene for antibiotic selection in eukaryotic cells. A gene of interest can be cloned into the multiple cloning site immediately downstream of the human cytomegalovirus (CMV) immediate early promoter. pLPCX also includes the Col E1 origin of replication and E.coli Amp+ gene for propagation and antibiotic selection in bacteria.

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