Annexin V-iFluor 488 + PI Apoptosis Detection Reagent/凋亡试剂盒价格_品牌:Chamot Biotech/乔默生物-丁香通官网

库存：充足

英文名：Annexin V-iFluor 488 + PI Apoptosis Detection Reagent kit

CAS号：N/A

保质期：2年

供应商：乔默生物技术（上海）有限公司

保存条件：4℃避光

规格：50 rxns/100 rxns/500 rxns/1000 rxns/2000 rxns/5000 rxns

Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露

的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料 iFluor 488 ( E x : 4 8 8 n m , E m : 5 1 4 n m ) ，

利用流式细胞仪或荧光显微镜可检测细胞凋亡。PI 是一种细胞核染料，搭配 Annexin V 使用可将处于不同凋亡

时期的细胞区分开。

产品应用

本试剂盒可应用于培养细胞凋亡检测（不推荐用于检测组织样本）

流式细胞实验操作步骤：

1.悬浮细胞离心（2000 rpm 离心 5 分钟）收集；贴壁细胞用不含 EDTA 的胰酶消化收集。

2.用 PBS 洗涤细胞两次（2000 rpm 离心 5 分钟）收集 1-5×105细胞。

3.加入 500 μL 1×Binding Buffer 重悬细胞。

4.加入 5 μL Annexin V-iFluor 488 混匀后加入 5 μL PI 混匀，室温避光反应 15-20 分钟。

5.孵育后将样本置于冰上,准备进行流式检测。

引用文献：

Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis.Journal of Hematology & Oncology volume 10, Article number: 72 (2017).(IF 11.059)
p53 modulates the effect of ribosomal protein S6 kinase1 (S6K1) on cisplatin toxicity in chronic myeloid leukemia cells.Pharmacological Research. 119 (2017) 443–462.(IF 5.893)
Effect of Metformin Nanoparticle-Mediated Thioredoxin Interacting Protein Expression on Oxaliplatin-Induced Peripheral Neuralgia. J Nanosci Nanotechnol. 2020 Oct 1;20(10):6123-6132.(IF 1.354)
Platelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery. Drug Delivery. 2022, VOL. 29, NO. 1, 937–949. (IF 6.419)
Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING. Frontiers in Pharmacology. 2022 Mar 3;13:837784. (IF 5.810)

该产品被引用文献

Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis.Journal of Hematology & Oncology volume 10, Article number: 72 (2017).(IF 11.059)
p53 modulates the effect of ribosomal protein S6 kinase1 (S6K1) on cisplatin toxicity in chronic myeloid leukemia cells.Pharmacological Research. 119 (2017) 443–462.(IF 5.893)
Effect of Metformin Nanoparticle-Mediated Thioredoxin Interacting Protein Expression on Oxaliplatin-Induced Peripheral Neuralgia. J Nanosci Nanotechnol. 2020 Oct 1;20(10):6123-6132.(IF 1.354)