eIF2α Antibody价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：eIF2α Antibody

抗原：synthetic peptide corresponding to the carboxy-terminal sequence of eIF2α

应用范围：W

宿主：Rabbit

供应商：CST

级别：详见MSDS文件

保质期：详见说明书

库存：大量

适应物种：H,M,R,Mk

是否单克隆：2

保存条件：-20°c

规格：100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R Mk	Endogenous	38	Rabbit

Protocols	9722: Western Blotting
Specificity / Sensitivity	eIF2α Antibody detects endogenous levels of total eIF2α protein.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of eIF2α. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM) using Phospho-eIF2α (Ser51) Antibody #9721 (upper) and control eIF2α Antibody (lower).
Background	Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.
Application References	Nanbo, A. et al. (2002) Epstein-Barr virus RNA confers resistance to interferon-alpha-induced apoptosis in Burkitt's lymphoma. EMBO J. 21, 954-965. Applications: Western Blotting Koumenis, C. et al. (2002) Regulation of protein synthesis by hypoxia via activation of the endoplasmic reticulum kinase PERK and phosphorylation of the translation initiation factor eIF2alpha. Mol. Cell. Biol. 22, 7405-7416. Applications: Western Blotting Chang, R. C. et al. (2002) Involvement of double-stranded RNA-dependent protein kinase and phosphorylation of eukaryotic initiation factor-2alpha in neuronal degeneration. J. Neurochem. 83, 1215-1225. Applications: Western Blotting Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products	2103 eIF2α (L57A5) Mouse mAb 3597 Phospho-eIF2α (Ser51) (119A11) Rabbit mAb 9721 Phospho-eIF2α (Ser51) Antibody 9918 Translational Control Antibody Sampler Kit 3302 GCN2 Antibody 3595 eIF2B-ε Antibody 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide For Research Use Only. Not For Use In Diagnostic Procedures.