APC Antibody价格_品牌:Cell Signaling Technology-丁香通官网

抗体英文名：APC Antibody

抗原：synthetic peptide corresponding to the sequence of human APC

应用范围：W, IP

宿主：Rabbit

保质期：详见说明书

级别：详见MSDS文件

库存：大量

供应商：CST

适应物种：H,M,R,Mk

是否单克隆：2

保存条件：-20°c

规格：100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H (M) (R) (Mk)	Endogenous	160 truncated APC in SW480 cells. 310 full-length APC.	Rabbit

Protocols	2504: Immunoprecipitation, Western Blotting
Specificity / Sensitivity	APC Antibody detects endogenous levels of total APC protein.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis (lane 1) and Immunoprecipitation (lanes 2-3) of extracts from SW480 cells. APC was immunoprecipitated with APC Antibody (lane 2) or with rabbit IgG (lane 3) to confirm specificity. Western blot was detection was performed using APC Antibody.
Background	The Adenomatous Polyposis Coli (APC) tumor suppressor gene is mutated in most familial and sporadic colorectal cancers and encodes a large cytoplasmic protein that is implicated in cell migration, cell adhesion, and proliferation (1). APC binds directly to microtubules and lack of APC leads to defective mitotic spindles and aneuploidy due to missegregation of chromosomes (2). APC is well characterized as a scaffolding protein, binds to β-catenin, and is involved in the regulation of its intracellular concentration. In the absence of a Wnt signal, GSK-3β phosphorylates all three members of the APC-β-catenin-axin complex and this phosphorylation of β-catenin creates a recognition site for ubiquitin, the signal for proteasome-mediated degradation. In the presence of a Wnt signal, dishevelled inactivates GSK-3β and β-catenin coordinates gene transcription of proteins important for the control of cell cycle progression and proliferation, such as cyclin D1 and c-Myc (3). Shih, I. M. et al. (2000) Cancer Res. 60, 1671-1676. Kaplan, K. B. et al. (2001) Nat Cell Biol. 3, 429-432. Fodde, R. (2002) Eur J Cancer. 38, 86-871.
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