His-Tag (27E8) Mouse mAb

价格：询价

品牌：Cell Signaling Technology 品牌认证

货号：2366

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抗体英文名：His-Tag (27E8) Mouse mAb

抗原：6xHis synthetic peptide

应用范围：W, IP

保质期：详见说明书

供应商：CST

级别：详见MSDS文件

库存：大量

适应物种：All

是否单克隆：1

保存条件：-20°c

规格：40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Applications	Reactivity	Sensitivity	Isotype
W IP	All	Transfected Only	Mouse IgG1

Protocols	2366: Immunoprecipitation, Western Blotting
Specificity / Sensitivity	His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide. Western Blotting Western blot analysis of extracts from control (lane 1) or cells expressing C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb. IP Immunoprecipitation of control lysate (lane 1) or C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb, followed by Western analysis with His-Tag Polyclonal Antibody #2365.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag cosisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1).As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1). Kroll, D.J. et al. (1993) DNA Cell Biol. 12, 441-453. di Guan, C. et al. (1988) Gene 67, 21-30.
Application References	Rishi, A. K. et al. (2003) Identification and characterization of a cell cycle and apoptosis regulatory protein-1 as a novel mediator of apoptosis signaling by retinoid CD437. J. Biol. Chem. 278, 33422-33435. Applications: IC-ABC Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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